Noninvasive fluorescent imaging reliably estimates biomass in vivo

F. E. Diehn, N. G. Costouros, M. S. Miller, Andrew L Feldman, H. R. Alexander, K. C P Li, S. K. Libutti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Whole-body optical imaging of small animals has emerged as a powerful, user-friendly, and high-throughput tool for assaying molecular and cellular processes as they occur in vivo. As with any imaging method, the utility of such technology relies on its ability to provide quantitative, biologically meaningful information about the physiologic or pathologic process of interest. Here we used an animal tumor model to evaluate the extent of correlation between noninvasively measured fluorescence and more traditional measurements of biomass (tumor volume and tumor weight). C57/BL6 mice were injected subcutaneously with murine colon adenocarcinoma cells that were engineered to express GFP. Serial measurements of fluorescence intensities were performed with a macroscopic in vivo fluorescence system. The progressive increases in intensity correlated strongly with growth in tumor volume, as determined by caliper measurements (R2 = 0.99). A more stringent correlation was found between fluorescence intensity and tumor weight (R2 = 0.97) than between volume and weight (R2 = 0.89). In a treatment experiment using tumor necrosis factor-α, fluorescence intensity (but not tumor volume) was able to differentiate between treated and control groups on day 1 post-treatment. These results validate the ability of noninvasive fluorescent imaging to quantify the number of viable, fluorescent cells in vivo.

Original languageEnglish (US)
Pages (from-to)1250-1255
Number of pages6
JournalBioTechniques
Volume33
Issue number6
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Tumor Burden
Biomass
Tumors
Fluorescence
Imaging techniques
Animals
Whole Body Imaging
Optical Imaging
Pathologic Processes
Colon
Adenocarcinoma
Animal Models
Tumor Necrosis Factor-alpha
Technology
Weights and Measures
Control Groups
Throughput
Growth
Neoplasms
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Diehn, F. E., Costouros, N. G., Miller, M. S., Feldman, A. L., Alexander, H. R., Li, K. C. P., & Libutti, S. K. (2002). Noninvasive fluorescent imaging reliably estimates biomass in vivo. BioTechniques, 33(6), 1250-1255.

Noninvasive fluorescent imaging reliably estimates biomass in vivo. / Diehn, F. E.; Costouros, N. G.; Miller, M. S.; Feldman, Andrew L; Alexander, H. R.; Li, K. C P; Libutti, S. K.

In: BioTechniques, Vol. 33, No. 6, 01.12.2002, p. 1250-1255.

Research output: Contribution to journalArticle

Diehn, FE, Costouros, NG, Miller, MS, Feldman, AL, Alexander, HR, Li, KCP & Libutti, SK 2002, 'Noninvasive fluorescent imaging reliably estimates biomass in vivo', BioTechniques, vol. 33, no. 6, pp. 1250-1255.
Diehn FE, Costouros NG, Miller MS, Feldman AL, Alexander HR, Li KCP et al. Noninvasive fluorescent imaging reliably estimates biomass in vivo. BioTechniques. 2002 Dec 1;33(6):1250-1255.
Diehn, F. E. ; Costouros, N. G. ; Miller, M. S. ; Feldman, Andrew L ; Alexander, H. R. ; Li, K. C P ; Libutti, S. K. / Noninvasive fluorescent imaging reliably estimates biomass in vivo. In: BioTechniques. 2002 ; Vol. 33, No. 6. pp. 1250-1255.
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