Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells

Federico Franchi, Martin G Rodriguez-Porcel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recently, molecular imaging has become a conditio sine qua non for cell-based regenerative medicine. Developments in molecular imaging techniques, such as reporter gene technology, have increasingly enabled the noninvasive assessment of the fate and biology of cells after cardiovascular applications. In this context, bioluminescence imaging is the most commonly used imaging modality in small animal models of preclinical studies. Here, we present a detailed protocol of a reporter gene imaging approach for monitoring the viability and biology of Mesenchymal Stem Cells transplanted in a mouse model of myocardial ischemia reperfusion injury.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages227-239
Number of pages13
Volume1553
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1553
ISSN (Print)10643745

Fingerprint

Molecular Imaging
Mesenchymal Stromal Cells
Reporter Genes
Cell Biology
Myocardial Reperfusion Injury
Regenerative Medicine
Reperfusion Injury
Myocardial Ischemia
Animal Models
Technology

Keywords

  • Bioluminescence
  • Heart
  • Luciferase
  • Molecular imaging
  • Monitoring
  • Reporter gene
  • Stem cells
  • Tracking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Franchi, F., & Rodriguez-Porcel, M. G. (2017). Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. In Methods in Molecular Biology (Vol. 1553, pp. 227-239). (Methods in Molecular Biology; Vol. 1553). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6756-8_18

Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. / Franchi, Federico; Rodriguez-Porcel, Martin G.

Methods in Molecular Biology. Vol. 1553 Humana Press Inc., 2017. p. 227-239 (Methods in Molecular Biology; Vol. 1553).

Research output: Chapter in Book/Report/Conference proceedingChapter

Franchi, F & Rodriguez-Porcel, MG 2017, Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. in Methods in Molecular Biology. vol. 1553, Methods in Molecular Biology, vol. 1553, Humana Press Inc., pp. 227-239. https://doi.org/10.1007/978-1-4939-6756-8_18
Franchi F, Rodriguez-Porcel MG. Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. In Methods in Molecular Biology. Vol. 1553. Humana Press Inc. 2017. p. 227-239. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6756-8_18
Franchi, Federico ; Rodriguez-Porcel, Martin G. / Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. Methods in Molecular Biology. Vol. 1553 Humana Press Inc., 2017. pp. 227-239 (Methods in Molecular Biology).
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