Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells

Federico Franchi, Martin G Rodriguez-Porcel

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Recently, molecular imaging has become a conditio sine qua non for cell-based regenerative medicine. Developments in molecular imaging techniques, such as reporter gene technology, have increasingly enabled the noninvasive assessment of the fate and biology of cells after cardiovascular applications. In this context, bioluminescence imaging is the most commonly used imaging modality in small animal models of preclinical studies. Here, we present a detailed protocol of a reporter gene imaging approach for monitoring the viability and biology of Mesenchymal Stem Cells transplanted in a mouse model of myocardial ischemia reperfusion injury.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages227-239
Number of pages13
Volume1553
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1553
ISSN (Print)10643745

Keywords

  • Bioluminescence
  • Heart
  • Luciferase
  • Molecular imaging
  • Monitoring
  • Reporter gene
  • Stem cells
  • Tracking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Franchi, F., & Rodriguez-Porcel, M. G. (2017). Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells. In Methods in Molecular Biology (Vol. 1553, pp. 227-239). (Methods in Molecular Biology; Vol. 1553). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6756-8_18