Non-viral gene delivery nanoparticles based on Poly(β-amino esters) for treatment of glioblastoma

Stephany Y. Tzeng, Hugo Guerrero Cazares, Elliott E. Martinez, Joel C. Sunshine, Alfredo Quinones-Hinojosa, Jordan J. Green

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Glioblastoma (GB) is currently characterized by low survival rates and therapies with insufficient efficacy. Here, we describe biodegradable polymers that can deliver genes to primary GB cells as well as GB tumor stem cells in vitro with low non-specific toxicity and transfection efficiencies of up to 60.6 ± 5% in normal (10%) serum conditions. We developed polymer-DNA nanoparticles that remained more stable in normal serum and could also be stored for at least 3 months in ready-to-use form with no measurable decrease in efficacy, expanding their potential in a practical or clinical setting. A subset of polymers was identified that shows a high degree of specificity to tumor cells compared with healthy astrocytes and human neural stem cells when cultured (separately or in co-culture), yielding higher transfection in GB cells while having little to no apparent effect on healthy cells.

Original languageEnglish (US)
Pages (from-to)5402-5410
Number of pages9
JournalBiomaterials
Volume32
Issue number23
DOIs
StatePublished - Aug 2011
Externally publishedYes

Fingerprint

Glioblastoma
Stem cells
Nanoparticles
Tumors
Esters
Polymers
Genes
Cells
Biodegradable polymers
Cell culture
Transfection
Toxicity
DNA
Neural Stem Cells
Neoplastic Stem Cells
Coculture Techniques
Serum
Astrocytes
Neoplasms
Therapeutics

Keywords

  • Cancer
  • DNA
  • Drug delivery
  • Gene therapy
  • Nanoparticle
  • Stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Non-viral gene delivery nanoparticles based on Poly(β-amino esters) for treatment of glioblastoma. / Tzeng, Stephany Y.; Guerrero Cazares, Hugo; Martinez, Elliott E.; Sunshine, Joel C.; Quinones-Hinojosa, Alfredo; Green, Jordan J.

In: Biomaterials, Vol. 32, No. 23, 08.2011, p. 5402-5410.

Research output: Contribution to journalArticle

Tzeng, Stephany Y. ; Guerrero Cazares, Hugo ; Martinez, Elliott E. ; Sunshine, Joel C. ; Quinones-Hinojosa, Alfredo ; Green, Jordan J. / Non-viral gene delivery nanoparticles based on Poly(β-amino esters) for treatment of glioblastoma. In: Biomaterials. 2011 ; Vol. 32, No. 23. pp. 5402-5410.
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