TY - JOUR
T1 - Non-random genetic alterations in the cyanobacterium Nostoc sp. exposed to space conditions
AU - Liu, Yuguang
AU - Jeraldo, Patricio
AU - Herbert, William
AU - McDonough, Samantha
AU - Eckloff, Bruce
AU - de Vera, Jean Pierre
AU - Cockell, Charles
AU - Leya, Thomas
AU - Baqué, Mickael
AU - Jen, Jin
AU - Schulze-Makuch, Dirk
AU - Walther-Antonio, Marina
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Understanding the impact of long-term exposure of microorganisms to space is critical in understanding how these exposures impact the evolution and adaptation of microbial life under space conditions. In this work we subjected Nostoc sp. CCCryo 231-06, a cyanobacterium capable of living under many different ecological conditions, and also surviving in extreme ones, to a 23-month stay at the International Space Station (the Biology and Mars Experiment, BIOMEX, on the EXPOSE-R2 platform) and returned it to Earth for single-cell genome analysis. We used microfluidic technology and single cell sequencing to identify the changes that occurred in the whole genome of single Nostoc cells. The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions. The cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.
AB - Understanding the impact of long-term exposure of microorganisms to space is critical in understanding how these exposures impact the evolution and adaptation of microbial life under space conditions. In this work we subjected Nostoc sp. CCCryo 231-06, a cyanobacterium capable of living under many different ecological conditions, and also surviving in extreme ones, to a 23-month stay at the International Space Station (the Biology and Mars Experiment, BIOMEX, on the EXPOSE-R2 platform) and returned it to Earth for single-cell genome analysis. We used microfluidic technology and single cell sequencing to identify the changes that occurred in the whole genome of single Nostoc cells. The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions. The cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.
UR - http://www.scopus.com/inward/record.url?scp=85134576108&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85134576108&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-16789-w
DO - 10.1038/s41598-022-16789-w
M3 - Article
AN - SCOPUS:85134576108
SN - 2045-2322
VL - 12
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 12580
ER -