Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer- patient tumorgraft progression in nude mice

Atsushi Suetsugu, Matthew Katz, Jason Fleming, Mark Truty, Ryan Thomas, Shigetoyo Saji, Hisataka Moriwaki, Michael Bouvet, Robert M. Hoffman

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

In order to individualize and therefore have more effective treatment for pancreatic cancer, we have developed a multicolor, imageable, orthotopic mouse model for individual patients with pancreatic cancer by passaging their tumors through transgenic nude mice expressing green fluorescent protein (GFP) and red fluorescent protein (RFP). The tumors acquired brightly fluorescent stroma from the transgenic host mice, which was stably associated with the tumors through multiple passages. In the present study, pancreatic cancer patient tumor specimens were initially established in NOD.CB17-Prkdcscid/NcrCrl (NOD/SCID) mice. The tumors were then passaged orthotopically into transgenic nude mice ubiquitously expressing GFP and subsequently to nude mice ubiquitously expressing RFP. The tumors, with very bright GFP and RFP stroma, were then orthotopically passaged to non-transgenic nude mice. It was possible to image the brightly fluorescent tumors non-invasively longitudinally as they progressed in the non-transgenic nude mice. This non-invasive imageable tumorgraft model will be valuable to screen for effective treatment options for individual patients with pancreatic cancer, as well as for the discovery of improved agents for this treatment-resistant disease.

Original languageEnglish (US)
Pages (from-to)3063-3067
Number of pages5
JournalAnticancer research
Volume32
Issue number8
StatePublished - Aug 2012

Keywords

  • GFP
  • Human patients
  • NOD/SCID mice
  • Non-invasive imaging
  • Nude mice
  • Pancreatic cancer
  • RFP
  • Stroma
  • Transgenic nude mice
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., & Hoffman, R. M. (2012). Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer- patient tumorgraft progression in nude mice. Anticancer research, 32(8), 3063-3067.