Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study

Cindy M. Chang; Jane C. Schroeder; Wen Yi Huang; Cherie H. Dunphy; Ralph S. Baric; Andrew F. Olshan; Kathleen C. Dorsey; Georgette A. Dent; James R. Cerhan; Charles F. Lynch; Nathaniel Rothman; Kenneth P. Cantor; Aaron Blair

doi:10.1016/j.leukres.2009.05.007

Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study

Cindy M. Chang, Jane C. Schroeder, Wen Yi Huang, Cherie H. Dunphy, Ralph S. Baric, Andrew F. Olshan, Kathleen C. Dorsey, Georgette A. Dent, James R. Cerhan, Charles F. Lynch, Nathaniel Rothman, Kenneth P. Cantor, Aaron Blair

Quantitative Health Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.

Original language	English (US)
Pages (from-to)	190-195
Number of pages	6
Journal	Leukemia Research
Volume	34
Issue number	2
DOIs	https://doi.org/10.1016/j.leukres.2009.05.007
State	Published - Feb 2010

Keywords

FISH
Lymphoma
Translocation
bcl-2
t(14 ;18)

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2009.05.007

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Chang, C. M., Schroeder, J. C., Huang, W. Y., Dunphy, C. H., Baric, R. S., Olshan, A. F., Dorsey, K. C., Dent, G. A., Cerhan, J. R., Lynch, C. F., Rothman, N., Cantor, K. P., & Blair, A. (2010). Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study. Leukemia Research, 34(2), 190-195. https://doi.org/10.1016/j.leukres.2009.05.007

Chang, CM, Schroeder, JC, Huang, WY, Dunphy, CH, Baric, RS, Olshan, AF, Dorsey, KC, Dent, GA, Cerhan, JR, Lynch, CF, Rothman, N, Cantor, KP & Blair, A 2010, 'Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study', Leukemia Research, vol. 34, no. 2, pp. 190-195. https://doi.org/10.1016/j.leukres.2009.05.007

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title = "Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study",

abstract = "We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.",

keywords = "FISH, Lymphoma, Translocation, bcl-2, t(14 ;18)",

author = "Chang, {Cindy M.} and Schroeder, {Jane C.} and Huang, {Wen Yi} and Dunphy, {Cherie H.} and Baric, {Ralph S.} and Olshan, {Andrew F.} and Dorsey, {Kathleen C.} and Dent, {Georgette A.} and Cerhan, {James R.} and Lynch, {Charles F.} and Nathaniel Rothman and Cantor, {Kenneth P.} and Aaron Blair",

note = "Funding Information: This study was supported in part by the UNC Lineberger Comprehensive Cancer Center (Population Sciences Research Award), the National Institute of Environmental Health Sciences (P30ES10126), the National Institutes of Health, National Cancer Institute (R03 CA71617-01), and the NIH Intramural Research Program (Division of Cancer Epidemiology and Genetics, National Cancer Institute). Special thanks to Sophia Wang and Charles Rabkin for their helpful comments, David W. Cowan from the Lineberger Comprehensive Cancer Center Immunohistochemistry/Hybridization Core Laboratory for guidance with FISH assays; Stephen Oglesbee and Boyd Yount from UNC-Chapel Hill; and Paige Tolbert from Emory University. ",

year = "2010",

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doi = "10.1016/j.leukres.2009.05.007",

language = "English (US)",

volume = "34",

pages = "190--195",

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T2 - Utility of fluorescence in situ hybridization (FISH) in a case-control study

AU - Chang, Cindy M.

AU - Schroeder, Jane C.

AU - Huang, Wen Yi

AU - Dunphy, Cherie H.

AU - Baric, Ralph S.

AU - Olshan, Andrew F.

AU - Dorsey, Kathleen C.

AU - Dent, Georgette A.

AU - Cerhan, James R.

AU - Lynch, Charles F.

AU - Rothman, Nathaniel

AU - Cantor, Kenneth P.

AU - Blair, Aaron

N1 - Funding Information: This study was supported in part by the UNC Lineberger Comprehensive Cancer Center (Population Sciences Research Award), the National Institute of Environmental Health Sciences (P30ES10126), the National Institutes of Health, National Cancer Institute (R03 CA71617-01), and the NIH Intramural Research Program (Division of Cancer Epidemiology and Genetics, National Cancer Institute). Special thanks to Sophia Wang and Charles Rabkin for their helpful comments, David W. Cowan from the Lineberger Comprehensive Cancer Center Immunohistochemistry/Hybridization Core Laboratory for guidance with FISH assays; Stephen Oglesbee and Boyd Yount from UNC-Chapel Hill; and Paige Tolbert from Emory University.

PY - 2010/2

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N2 - We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.

AB - We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.

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KW - Lymphoma

KW - Translocation

KW - bcl-2

KW - t(14 ;18)

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Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: Utility of fluorescence in situ hybridization (FISH) in a case-control study

Abstract

Keywords

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