No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan

Rika A. Furuta; Takayuki Miyazawa; Takeki Sugiyama; Hirohiko Kuratsune; Yasuhiro Ikeda; Eiji Sato; Naoko Misawa; Yasuhito Nakatomi; Ryuta Sakuma; Kazuta Yasui; Kouzi Yamaguti; Fumiya Hirayama

doi:10.1186/1742-4690-8-20

No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan

Rika A. Furuta, Takayuki Miyazawa, Takeki Sugiyama, Hirohiko Kuratsune, Yasuhiro Ikeda, Eiji Sato, Naoko Misawa, Yasuhito Nakatomi, Ryuta Sakuma, Kazuta Yasui, Kouzi Yamaguti, Fumiya Hirayama

Molecular Medicine

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Abstract

Background: The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan, we screened three populations--healthy donors (n = 500), patients with PC (n = 67), and patients with CFS (n = 100)--for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all (both antibody-positive and antibody-negative) patients with CFS for XMRV DNA.Results: Antibody screening by immunoblot analysis showed that a fraction of the cases (1.6-3.0%) possessed anti-Gag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein, but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients, we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further, all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells.Conclusion: Our data show no solid evidence of XMRV infection in any of the three populations tested, implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However, the lack of adequate human specimens as a positive control in Ab screening and the limited sample size do not allow us to draw a firm conclusion.

Original language	English (US)
Article number	20
Journal	Retrovirology
Volume	8
DOIs	https://doi.org/10.1186/1742-4690-8-20
State	Published - Mar 17 2011

ASJC Scopus subject areas

Virology
Infectious Diseases

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@article{de6318e4e80e416498bd6936aab5c500,

title = "No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan",

abstract = "Background: The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan, we screened three populations--healthy donors (n = 500), patients with PC (n = 67), and patients with CFS (n = 100)--for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all (both antibody-positive and antibody-negative) patients with CFS for XMRV DNA.Results: Antibody screening by immunoblot analysis showed that a fraction of the cases (1.6-3.0%) possessed anti-Gag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein, but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients, we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further, all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells.Conclusion: Our data show no solid evidence of XMRV infection in any of the three populations tested, implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However, the lack of adequate human specimens as a positive control in Ab screening and the limited sample size do not allow us to draw a firm conclusion.",

author = "Furuta, {Rika A.} and Takayuki Miyazawa and Takeki Sugiyama and Hirohiko Kuratsune and Yasuhiro Ikeda and Eiji Sato and Naoko Misawa and Yasuhito Nakatomi and Ryuta Sakuma and Kazuta Yasui and Kouzi Yamaguti and Fumiya Hirayama",

note = "Funding Information: This work was supported by grant-in-aid “virus-37-2007” from the Blood Service Headquarters, Japanese Red Cross Society (RAF) and by a grant from the Bio-oriented Technology Research Advancement Institution (TM). We thank Dr Robert H. Silverman (Cleveland Clinic, Cleveland, OH) for the pcDNA3.1-VP62 XMRV clone, Dr Dan R. Littman (New York University, New York, NY) for pAmpho, and Dr Akio Adachi (Tokushima University, Tokushima, Japan) for the pNL43 env-deleted mutant. We also thank Ms Mika Kagura for collecting the blood samples (Osaka City University, Osaka, Japan), Dr Takayuki Shojima (Kyoto University, Kyoto, Japan) for helping with the experiments, and our colleagues Mr Hideki Aso and Mr Masaki Yasui (Department of Blood Products, Osaka Red Cross Blood Center, Osaka, Japan) for shipping the test blood samples weekly. Finally, we thank the Bio-oriented Technology Research Advancement Institution for technical advice.",

year = "2011",

month = mar,

day = "17",

doi = "10.1186/1742-4690-8-20",

language = "English (US)",

volume = "8",

journal = "Retrovirology",

issn = "1742-4690",

publisher = "BioMed Central",

}

TY - JOUR

T1 - No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan

AU - Furuta, Rika A.

AU - Miyazawa, Takayuki

AU - Sugiyama, Takeki

AU - Kuratsune, Hirohiko

AU - Ikeda, Yasuhiro

AU - Sato, Eiji

AU - Misawa, Naoko

AU - Nakatomi, Yasuhito

AU - Sakuma, Ryuta

AU - Yasui, Kazuta

AU - Yamaguti, Kouzi

AU - Hirayama, Fumiya

N1 - Funding Information: This work was supported by grant-in-aid “virus-37-2007” from the Blood Service Headquarters, Japanese Red Cross Society (RAF) and by a grant from the Bio-oriented Technology Research Advancement Institution (TM). We thank Dr Robert H. Silverman (Cleveland Clinic, Cleveland, OH) for the pcDNA3.1-VP62 XMRV clone, Dr Dan R. Littman (New York University, New York, NY) for pAmpho, and Dr Akio Adachi (Tokushima University, Tokushima, Japan) for the pNL43 env-deleted mutant. We also thank Ms Mika Kagura for collecting the blood samples (Osaka City University, Osaka, Japan), Dr Takayuki Shojima (Kyoto University, Kyoto, Japan) for helping with the experiments, and our colleagues Mr Hideki Aso and Mr Masaki Yasui (Department of Blood Products, Osaka Red Cross Blood Center, Osaka, Japan) for shipping the test blood samples weekly. Finally, we thank the Bio-oriented Technology Research Advancement Institution for technical advice.

PY - 2011/3/17

Y1 - 2011/3/17

N2 - Background: The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan, we screened three populations--healthy donors (n = 500), patients with PC (n = 67), and patients with CFS (n = 100)--for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all (both antibody-positive and antibody-negative) patients with CFS for XMRV DNA.Results: Antibody screening by immunoblot analysis showed that a fraction of the cases (1.6-3.0%) possessed anti-Gag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein, but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients, we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further, all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells.Conclusion: Our data show no solid evidence of XMRV infection in any of the three populations tested, implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However, the lack of adequate human specimens as a positive control in Ab screening and the limited sample size do not allow us to draw a firm conclusion.

AB - Background: The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan, we screened three populations--healthy donors (n = 500), patients with PC (n = 67), and patients with CFS (n = 100)--for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all (both antibody-positive and antibody-negative) patients with CFS for XMRV DNA.Results: Antibody screening by immunoblot analysis showed that a fraction of the cases (1.6-3.0%) possessed anti-Gag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein, but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients, we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further, all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells.Conclusion: Our data show no solid evidence of XMRV infection in any of the three populations tested, implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However, the lack of adequate human specimens as a positive control in Ab screening and the limited sample size do not allow us to draw a firm conclusion.

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U2 - 10.1186/1742-4690-8-20

DO - 10.1186/1742-4690-8-20

M3 - Article

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AN - SCOPUS:79952693766

SN - 1742-4690

VL - 8

JO - Retrovirology

JF - Retrovirology

M1 - 20

ER -

No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan

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