NMR microscopy of tissue in organic and mixed solvents

Slobodan I Macura, Prasanna K. Mishra, Jeffrey D. Gamez, Istvan Pirko

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The use organic and mixed solvents for nuclear magnetic resonance microscopy of fixed tissue is proposed as a means for improving image information content. NMR properties of some standard solvents (methanol, acetone or DMSO) and solvents in use for tissue processing in pathology (xylenes, paraffin, "Clearify") have been measured, reviewed and analyzed. It was found that DMSO and paraffin are very useful solvents that provide images of better quality than those obtained in water (neutralized formalin buffer). This is illustrated on formalin-fixed mouse brain sections imaged at 16.4 T (700 MHz).

Original languageEnglish (US)
Pages (from-to)1641-1654
Number of pages14
JournalJournal of the Serbian Chemical Society
Volume78
Issue number11
DOIs
StatePublished - 2013

Fingerprint

Microscopic examination
Nuclear magnetic resonance
Tissue
Dimethyl Sulfoxide
Paraffin
Formaldehyde
Xylenes
Pathology
Acetone
Methanol
Brain
Buffers
Water
Processing

Keywords

  • Cross-relaxation
  • Magnetization transfer
  • MRI
  • MRM
  • NMR
  • NMR imaging
  • NMR microscopy
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

NMR microscopy of tissue in organic and mixed solvents. / Macura, Slobodan I; Mishra, Prasanna K.; Gamez, Jeffrey D.; Pirko, Istvan.

In: Journal of the Serbian Chemical Society, Vol. 78, No. 11, 2013, p. 1641-1654.

Research output: Contribution to journalArticle

Macura, Slobodan I ; Mishra, Prasanna K. ; Gamez, Jeffrey D. ; Pirko, Istvan. / NMR microscopy of tissue in organic and mixed solvents. In: Journal of the Serbian Chemical Society. 2013 ; Vol. 78, No. 11. pp. 1641-1654.
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