Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

Bruno M. Balsiger; Judith A. Duenes; Noriya Obtani; Chikashi Shibata; Gianrico Farrugia; William J. Anding; Michael G. Sarr

doi:10.1016/S1091-255X(00)80037-2

Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

Bruno M. Balsiger, Judith A. Duenes, Noriya Obtani, Chikashi Shibata, Gianrico Farrugia, William J. Anding, Michael G. Sarr

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, N^G-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

Original language	English (US)
Pages (from-to)	86-92
Number of pages	7
Journal	Journal of Gastrointestinal Surgery
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/S1091-255X(00)80037-2
State	Published - 2000

Keywords

Inhibitory neurotransmitters
Motility
Nitric oxide
Small bowel transplantation
Smooth muscle

ASJC Scopus subject areas

Surgery
Gastroenterology

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10.1016/S1091-255X(00)80037-2

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title = "Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation",

abstract = "Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.",

keywords = "Inhibitory neurotransmitters, Motility, Nitric oxide, Small bowel transplantation, Smooth muscle",

author = "Balsiger, {Bruno M.} and Duenes, {Judith A.} and Noriya Obtani and Chikashi Shibata and Gianrico Farrugia and Anding, {William J.} and Sarr, {Michael G.}",

note = "Funding Information: Previous work from our laboratory has investigated the early and late effects of SBT on contractile activity of circular smooth muscle of the rat ileum. Al- though we found an impressive adrenergic hypersensitivity after SBT, spontaneous contractile activity remained unchanged. 3,4 When examined by exogenous application of nitric oxide (NO) and NO antagonists and during intrinsic neural excitation by electrical field stimulation (EFS), inhibitory neurotransmission to ileal circular muscle appeared to be independent of NO. 5 In contrast, in jejunal circular muscle, SBT induced an increase in spontaneous contractile activity and augmented EFS-induced nonadrenergic, non-cholinergic (NANC) inhibition. Because no changes Supported by grant DK 39337 from the National Institutes of Health, United States Public Health Service, the Mayo Foundation, the Swiss National Foundation, and the Department of Visceral and Transplantation Surgery, University of Bern, Switzerland. Part of this study was presented at a poster session at the Fortieth Annual Meeting of The Society for Surgery of the Alimentary Tract, Orlando, Fla., May 16-19, 1999. An abstract of this work was published in Gastroenterology1 16:A1349, 1999. Correspondence: Michael G. Sarr, M.D., Professor of Surgery, Gastroenterology Research Unit, Mayo Clinic, 200 First Street SW, Rochester, MN 55905.",

year = "2000",

doi = "10.1016/S1091-255X(00)80037-2",

language = "English (US)",

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pages = "86--92",

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T1 - Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

AU - Balsiger, Bruno M.

AU - Duenes, Judith A.

AU - Obtani, Noriya

AU - Shibata, Chikashi

AU - Farrugia, Gianrico

AU - Anding, William J.

AU - Sarr, Michael G.

N1 - Funding Information: Previous work from our laboratory has investigated the early and late effects of SBT on contractile activity of circular smooth muscle of the rat ileum. Al- though we found an impressive adrenergic hypersensitivity after SBT, spontaneous contractile activity remained unchanged. 3,4 When examined by exogenous application of nitric oxide (NO) and NO antagonists and during intrinsic neural excitation by electrical field stimulation (EFS), inhibitory neurotransmission to ileal circular muscle appeared to be independent of NO. 5 In contrast, in jejunal circular muscle, SBT induced an increase in spontaneous contractile activity and augmented EFS-induced nonadrenergic, non-cholinergic (NANC) inhibition. Because no changes Supported by grant DK 39337 from the National Institutes of Health, United States Public Health Service, the Mayo Foundation, the Swiss National Foundation, and the Department of Visceral and Transplantation Surgery, University of Bern, Switzerland. Part of this study was presented at a poster session at the Fortieth Annual Meeting of The Society for Surgery of the Alimentary Tract, Orlando, Fla., May 16-19, 1999. An abstract of this work was published in Gastroenterology1 16:A1349, 1999. Correspondence: Michael G. Sarr, M.D., Professor of Surgery, Gastroenterology Research Unit, Mayo Clinic, 200 First Street SW, Rochester, MN 55905.

PY - 2000

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N2 - Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

AB - Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

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KW - Smooth muscle

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Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

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