Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

Bruno M. Balsiger, Judith A. Duenes, Noriya Obtani, Chikashi Shibata, Gianrico Farrugia, William J. Anding, Michael G. Sarr

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume4
Issue number1
StatePublished - Jan 2000

Fingerprint

Jejunum
Nitric Oxide
Transplantation
Muscles
Electric Stimulation
Nitric Oxide Synthase
omega-N-Methylarginine
Methylene Blue
Smooth Muscle
Neurotransmitter Agents
Anesthesia
Salts

Keywords

  • Inhibitory neurotransmitters
  • Motility
  • Nitric oxide
  • Small bowel transplantation
  • Smooth muscle

ASJC Scopus subject areas

  • Surgery

Cite this

Balsiger, B. M., Duenes, J. A., Obtani, N., Shibata, C., Farrugia, G., Anding, W. J., & Sarr, M. G. (2000). Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation. Journal of Gastrointestinal Surgery, 4(1), 86-92.

Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation. / Balsiger, Bruno M.; Duenes, Judith A.; Obtani, Noriya; Shibata, Chikashi; Farrugia, Gianrico; Anding, William J.; Sarr, Michael G.

In: Journal of Gastrointestinal Surgery, Vol. 4, No. 1, 01.2000, p. 86-92.

Research output: Contribution to journalArticle

Balsiger, BM, Duenes, JA, Obtani, N, Shibata, C, Farrugia, G, Anding, WJ & Sarr, MG 2000, 'Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation', Journal of Gastrointestinal Surgery, vol. 4, no. 1, pp. 86-92.
Balsiger, Bruno M. ; Duenes, Judith A. ; Obtani, Noriya ; Shibata, Chikashi ; Farrugia, Gianrico ; Anding, William J. ; Sarr, Michael G. / Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation. In: Journal of Gastrointestinal Surgery. 2000 ; Vol. 4, No. 1. pp. 86-92.
@article{8fd76908cfbf4cfe8c16ead756d55afc,
title = "Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation",
abstract = "Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.",
keywords = "Inhibitory neurotransmitters, Motility, Nitric oxide, Small bowel transplantation, Smooth muscle",
author = "Balsiger, {Bruno M.} and Duenes, {Judith A.} and Noriya Obtani and Chikashi Shibata and Gianrico Farrugia and Anding, {William J.} and Sarr, {Michael G.}",
year = "2000",
month = "1",
language = "English (US)",
volume = "4",
pages = "86--92",
journal = "Journal of Gastrointestinal Surgery",
issn = "1091-255X",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Nitric Oxide Pathways in Circular Muscle of the Rat Jejunum before and after Small Bowel Transplantation

AU - Balsiger, Bruno M.

AU - Duenes, Judith A.

AU - Obtani, Noriya

AU - Shibata, Chikashi

AU - Farrugia, Gianrico

AU - Anding, William J.

AU - Sarr, Michael G.

PY - 2000/1

Y1 - 2000/1

N2 - Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

AB - Previous studies suggest that nitric oxide synthase is upregulated after small bowel transplantation which may have implications in enteric dysfunction after small bowel transplantation. The aim of this study was to determine the role of nitric oxide in nonadrenergic, noncholinergic inhibitory function after small bowel transplantation in rat jejunal circular muscle. The following four groups of rats (n = ≥8 rats per group) were studied: Neurally intact control animals; 1 week after anesthesia and sham celiotomy, and either 1 week or 8 weeks after isogeneic, orthotopic small bowel transplantation. Full-thickness jejunal circular muscle strips were evaluated under isometric conditions for spontaneous contractile activity, response to electrical field stimulation, and effects of exogenous nitric oxide and nitric oxide antagonists. Spontaneous activity did not differ among groups. Electrical field stimulation inhibited activity similarly in all groups. Exogenous nitric oxide, NG-monomethyl L-arginine monoacetate salt (a nitric oxide synthase inhibitor), and methylene blue (cGMP antagonist) had no effect on spontaneous activity. Neither nitric oxide antagonist altered the inhibitory response to neural excitation by electrical field stimulation in any group. Nitric oxide, a known inhibitory neurotransmitter in other gut smooth muscle, has no apparent role in rat jejunal circular muscle before or after small bowel transplantation.

KW - Inhibitory neurotransmitters

KW - Motility

KW - Nitric oxide

KW - Small bowel transplantation

KW - Smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=0033629013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033629013&partnerID=8YFLogxK

M3 - Article

C2 - 10631367

AN - SCOPUS:0033629013

VL - 4

SP - 86

EP - 92

JO - Journal of Gastrointestinal Surgery

JF - Journal of Gastrointestinal Surgery

SN - 1091-255X

IS - 1

ER -