Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle

Leo M A Heunks, Mark J. Cody, Paige C. Geiger, P. N Richard Dekhuijzen, Gary C Sieck

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The effects of the nitric oxide (NO) donor spermine NONOate (Sp-NO, 1.0 mM) on cross-bridge recruitment and cross-bridge cycling kinetics were studied in permeabilized rabbit psoas muscle fibers. Fibers were activated at various Ca2+ concentrations (pCa, negative logarithm of Ca2+ concentration), and the pCa at which force was maximal (pCa 4.0) and ∼50% of maximal (pCa50 5.6) were determined. Fiber stiffness was determined using 1-kHz sinusoidal length perturbations, and the fraction of cross bridges in the force-generating state was estimated by the ratio of stiffness during maximal (pCa 4.0) and submaximal (pCa 5.6) Ca2+ activation to stiffness during rigor (at pCa 4.0). Cross-bridge cycling kinetics were evaluated by measuring the rate constant for force redevelopment after quick release (by 15% of optimal fiber length, Lo) and restretch of the fiber to Lo. Exposing fibers to Sp-NO for 10 min reduced force and the fraction of cross bridges in the force-generating state at maximal and submaximal (pCa50) Ca2+ activation. However, the effects of Sp-NO were more pronounced during submaximal Ca2+ activation. Sp-NO also reduced the rate constant for force redevelopment but only during submaximal Ca2+ activation. We conclude that Sp-NO reduces Ca2+ sensitivity by decreasing the number of cross bridges in the strongly bound state and also impairs cross-bridge cycling kinetics during submaximal activation.

Original languageEnglish (US)
Pages (from-to)2233-2239
Number of pages7
JournalJournal of Applied Physiology
Volume91
Issue number5
StatePublished - 2001

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Nitric Oxide
Skeletal Muscle
Psoas Muscles
Nitric Oxide Donors
Rabbits

Keywords

  • Rabbit psoas
  • Rate of force redevelopment
  • Spermine NONOate
  • Stiffness

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Heunks, L. M. A., Cody, M. J., Geiger, P. C., Dekhuijzen, P. N. R., & Sieck, G. C. (2001). Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle. Journal of Applied Physiology, 91(5), 2233-2239.

Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle. / Heunks, Leo M A; Cody, Mark J.; Geiger, Paige C.; Dekhuijzen, P. N Richard; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 91, No. 5, 2001, p. 2233-2239.

Research output: Contribution to journalArticle

Heunks, LMA, Cody, MJ, Geiger, PC, Dekhuijzen, PNR & Sieck, GC 2001, 'Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle', Journal of Applied Physiology, vol. 91, no. 5, pp. 2233-2239.
Heunks LMA, Cody MJ, Geiger PC, Dekhuijzen PNR, Sieck GC. Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle. Journal of Applied Physiology. 2001;91(5):2233-2239.
Heunks, Leo M A ; Cody, Mark J. ; Geiger, Paige C. ; Dekhuijzen, P. N Richard ; Sieck, Gary C. / Nitric oxide impairs Ca2+ activation and slows cross-bridge cycling kinetics in skeletal muscle. In: Journal of Applied Physiology. 2001 ; Vol. 91, No. 5. pp. 2233-2239.
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