Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture

H. J. Häuselmann, L. Oppliger, B. A. Michel, M. Stefanovic-Racic, Christopher H Evans

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Interleukin-1α and β induced the production of large amounts of nitric oxide by normal, human articular chondrocytes in alginate culture; at the same time the biosynthesis of proteoglycan was strongly suppressed. In a dose-dependent manner, NG-monomethyl-l-arginine both inhibited nitric oxide formation and relieved the suppression of proteoglycan synthesis. However concentrations of NG-monomethyl-l-arginine which completely prevented nitric oxide production only partially restored proteoglycan biosynthesis, even at low doses of interleukin-1 where suppression of proteoglycan synthesis was modest. The organic donor of nitric oxide, S-nitrosyl-acetyl-d,l- penicillamine also inhibited proteoglycan biosynthesis, but not as extensively as interleukin-1. These data suggest that interleukin-1 suppresses synthesis of the cartilaginous matrix through more than one mechanism, at least one of which is dependent upon the production of nitric oxide.

Original languageEnglish (US)
Pages (from-to)361-364
Number of pages4
JournalFEBS Letters
Volume352
Issue number3
DOIs
StatePublished - Oct 3 1994
Externally publishedYes

Fingerprint

Biosynthesis
Proteoglycans
Chondrocytes
Nitric Oxide
Interleukin-1
Joints
Arginine
Penicillamine
Nitric Oxide Donors
alginic acid

Keywords

  • Arthritis
  • Chondrocyte
  • Human articular cartilage
  • Interleukin-1
  • Nitric oxide
  • Proteoglycan synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture. / Häuselmann, H. J.; Oppliger, L.; Michel, B. A.; Stefanovic-Racic, M.; Evans, Christopher H.

In: FEBS Letters, Vol. 352, No. 3, 03.10.1994, p. 361-364.

Research output: Contribution to journalArticle

Häuselmann, H. J. ; Oppliger, L. ; Michel, B. A. ; Stefanovic-Racic, M. ; Evans, Christopher H. / Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture. In: FEBS Letters. 1994 ; Vol. 352, No. 3. pp. 361-364.
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