Nitric oxide and proteoglycan biosynthesis by human articular chondrocytes in alginate culture

H. J. Häuselmann, L. Oppliger, B. A. Michel, M. Stefanovic-Racic, C. H. Evans

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Interleukin-1α and β induced the production of large amounts of nitric oxide by normal, human articular chondrocytes in alginate culture; at the same time the biosynthesis of proteoglycan was strongly suppressed. In a dose-dependent manner, NG-monomethyl-l-arginine both inhibited nitric oxide formation and relieved the suppression of proteoglycan synthesis. However concentrations of NG-monomethyl-l-arginine which completely prevented nitric oxide production only partially restored proteoglycan biosynthesis, even at low doses of interleukin-1 where suppression of proteoglycan synthesis was modest. The organic donor of nitric oxide, S-nitrosyl-acetyl-d,l- penicillamine also inhibited proteoglycan biosynthesis, but not as extensively as interleukin-1. These data suggest that interleukin-1 suppresses synthesis of the cartilaginous matrix through more than one mechanism, at least one of which is dependent upon the production of nitric oxide.

Original languageEnglish (US)
Pages (from-to)361-364
Number of pages4
JournalFEBS Letters
Volume352
Issue number3
DOIs
StatePublished - Oct 3 1994

Keywords

  • Arthritis
  • Chondrocyte
  • Human articular cartilage
  • Interleukin-1
  • Nitric oxide
  • Proteoglycan synthesis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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