Nitric oxide and gallbladder motility in prairie dogs

Howard Salomons, Andrew P. Keaveny, Robert Henihan, Gwynneth Offner, Ashok Sengupta, Wayne W. Lamorte, Nezam H. Afdhal

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study we evaluated the role of nitric oxide (NO) on gallbladder motility in the normal prairie dog by 1) immunohistochemistry, 2) an enzymatic assay for NO synthase (NOS), and 3) an in vivo model to measure whole gallbladder tone and contractility. NOS was localized to gallbladder mucosal cells by NADPH-diaphorase and polyclonal antibodies to a constitutive brain NOS. Gallbladder mucosal homogenates demonstrated total NOS activity in the range of 578 ± 115 pmol · mg protein-1 · 30 min-1. Blockade of NOS activity in vivo using Nω-nitro-L-arginine methyl ester resulted in an up to 80% increase in gallbladder tone from basal. A 40% increase in tone was seen with methylene blue, suggesting that tone was maintained by both NO activation of guanylate cyclase and possibly direct effects on Ca2+ channels. An exogenous nitrosothiol, S-nitroso-N-acetyl-cysteine, abolished cholecystokinin (CCK) octapeptide and bethanechol-stimulated gallbladder contraction. We conclude that the prairie dog gallbladder contains constitutive NOS and synthesizes NO, which is important for the maintenance of basal gallbladder tone and is an inhibitor of the contractile response of the gallbladder to agonists such as CCK and bethanechol.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume272
Issue number4 35-4
StatePublished - Apr 1997
Externally publishedYes

Fingerprint

Sciuridae
Gallbladder
Nitric Oxide
Nitric Oxide Synthase
Bethanechol
NADPH Dehydrogenase
Sincalide
Guanylate Cyclase
Methylene Blue
Cholecystokinin
Enzyme Assays
Cysteine
Immunohistochemistry
Maintenance
Antibodies

Keywords

  • cholecystokinin
  • cholelithiasis
  • guanosine 3',5'-cyclic monophosphate
  • smooth muscle

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Salomons, H., Keaveny, A. P., Henihan, R., Offner, G., Sengupta, A., Lamorte, W. W., & Afdhal, N. H. (1997). Nitric oxide and gallbladder motility in prairie dogs. American Journal of Physiology - Gastrointestinal and Liver Physiology, 272(4 35-4).

Nitric oxide and gallbladder motility in prairie dogs. / Salomons, Howard; Keaveny, Andrew P.; Henihan, Robert; Offner, Gwynneth; Sengupta, Ashok; Lamorte, Wayne W.; Afdhal, Nezam H.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 272, No. 4 35-4, 04.1997.

Research output: Contribution to journalArticle

Salomons, H, Keaveny, AP, Henihan, R, Offner, G, Sengupta, A, Lamorte, WW & Afdhal, NH 1997, 'Nitric oxide and gallbladder motility in prairie dogs', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 272, no. 4 35-4.
Salomons H, Keaveny AP, Henihan R, Offner G, Sengupta A, Lamorte WW et al. Nitric oxide and gallbladder motility in prairie dogs. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 Apr;272(4 35-4).
Salomons, Howard ; Keaveny, Andrew P. ; Henihan, Robert ; Offner, Gwynneth ; Sengupta, Ashok ; Lamorte, Wayne W. ; Afdhal, Nezam H. / Nitric oxide and gallbladder motility in prairie dogs. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 ; Vol. 272, No. 4 35-4.
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