Nicotinic acid adenine dinucleotide phosphate: A new Ca2+ releasing agent in kidney

J. Cheng, A. N.K. Yusufi, M. A. Thompson, E. N. Chini, J. P. Grande

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Nicotinic acid adenine dinucleotide phosphate (NAADP), a molecule derived from β-NADP, has been shown to trigger Ca2+ release from intracellular stores of invertebrate eggs and mammalian cell microsomes. NAADP-induced Ca2+ release occurs through a mechanism distinct from that of inositol-1,4,5-trisphosphate- or cyclic ADP-ribose-elicited Ca2+ release. This study investigated whether NAADP can be synthesized in rat kidney. Extracts from glomeruli, mesangial cells, and papilla have high NAADP synthetic capacities. Conversely, synthesis of NAADP in kidney cortex was almost undetectable. Furthermore, 9-cis-retinoic acid significantly upregulated NAADP synthesis in mesangial cells. Authenticity of NAADP biosynthesis in glomeruli was affirmed by HPLC analysis. NAADP stimulated Ca2+ release from mesangial cell microsomes through a pathway distinct from that of inositol-1,4,5-trisphosphate or cyclic ADP-ribose. NAADP-triggered Ca2+ release may play an important role in regulation of renal function.

Original languageEnglish (US)
Pages (from-to)54-60
Number of pages7
JournalJournal of the American Society of Nephrology
Volume12
Issue number1
StatePublished - 2001

ASJC Scopus subject areas

  • Nephrology

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