Nicotinic acetylcholine receptors at the single-channel level

Cecilia Bouzat, Steven M Sine

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Over the past four decades, the patch clamp technique and nicotinic ACh (nACh) receptors have established an enduring partnership. Like all good partnerships, each partner has proven significant in its own right, while their union has spurred innumerable advances in life science research. A member and prototype of the superfamily of pentameric ligand-gated ion channels, the nACh receptor is a chemo-electric transducer, binding ACh released from nerves and rapidly opening its channel to cation flow to elicit cellular excitation. A subject of a Nobel Prize in Physiology or Medicine, the patch clamp technique provides unprecedented resolution of currents through single ion channels in their native cellular environments. Here, focusing on muscle and α7 nACh receptors, we describe the extraordinary contribution of the patch clamp technique towards understanding how they activate in response to neurotransmitter, how subtle structural and mechanistic differences among nACh receptor subtypes translate into significant physiological differences, and how nACh receptors are being exploited as therapeutic drug targets.

Original languageEnglish (US)
JournalBritish Journal of Pharmacology
DOIs
StateAccepted/In press - 2017

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Nicotinic Receptors
Cholinergic Receptors
Patch-Clamp Techniques
Nobel Prize
Ligand-Gated Ion Channels
Biological Science Disciplines
Transducers
Ion Channels
Neurotransmitter Agents
Cations
Medicine
Muscles
Research
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology

Cite this

Nicotinic acetylcholine receptors at the single-channel level. / Bouzat, Cecilia; Sine, Steven M.

In: British Journal of Pharmacology, 2017.

Research output: Contribution to journalArticle

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