Nicotine induces apoptosis in TM3 mouse Leydig cells

Khae Hawn Kim, Kwan Joong Joo, Heung Jae Park, Chil Hun Kwon, Mi-Hyeon D Jang, Chang Ju Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective: To investigate whether nicotine-induced testicular toxicity involves the induction of apoptosis in Leydig cells. Design: Cell study using cells of the TM3 cell line derived from mouse Leydig cells. Setting: Academic research laboratory. Intervention(s): Morphological and biochemical analyses for the detection of apoptosis. Main Outcome Measure(s): The effect of nicotine on the occurrence of apoptosis was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, 4,6-diamidino-2-phenylindole staining, terminal deoxynuclotidyl transferase-mediated dUTP nick end labeling assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction, caspase-3 enzyme assay, and Western blot analysis. Nicotine treatment exhibits several features of apoptosis in mouse Leydig cells. Result(s): TM3 cells treated with nicotine exhibit several features of apoptosis. It was also shown that nicotine increases the mRNA level of bax and decreases that of bcl-2. In addition, nicotine enhanced the expression of the activated form of caspase-3 and caspase-3 enzyme activity. Conclusion(s): Nicotine appears to activate specific intracellular death-related pathways, probably by bax-dependent activation of caspase-3, inducing apoptosis in Leydig cells. Thus, nicotine-induced apoptosis of Leydig cells might be one of the important mechanisms behind nicotine-related urogenital disorders in men.

Original languageEnglish (US)
Pages (from-to)1093-1099
Number of pages7
JournalFertility and Sterility
Volume83
Issue number4 SUPPL.
DOIs
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Leydig Cells
Nicotine
Apoptosis
Caspase 3
Enzyme Assays
DNA Fragmentation
Transferases
Reverse Transcription
Western Blotting
Outcome Assessment (Health Care)
Staining and Labeling
Cell Line
Polymerase Chain Reaction
Messenger RNA
Enzymes

Keywords

  • Apoptosis
  • Caspase-3
  • Mouse Leydig cells
  • Nicotine

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Kim, K. H., Joo, K. J., Park, H. J., Kwon, C. H., Jang, M-H. D., & Kim, C. J. (2005). Nicotine induces apoptosis in TM3 mouse Leydig cells. Fertility and Sterility, 83(4 SUPPL.), 1093-1099. https://doi.org/10.1016/j.fertnstert.2004.12.013

Nicotine induces apoptosis in TM3 mouse Leydig cells. / Kim, Khae Hawn; Joo, Kwan Joong; Park, Heung Jae; Kwon, Chil Hun; Jang, Mi-Hyeon D; Kim, Chang Ju.

In: Fertility and Sterility, Vol. 83, No. 4 SUPPL., 04.2005, p. 1093-1099.

Research output: Contribution to journalArticle

Kim, KH, Joo, KJ, Park, HJ, Kwon, CH, Jang, M-HD & Kim, CJ 2005, 'Nicotine induces apoptosis in TM3 mouse Leydig cells', Fertility and Sterility, vol. 83, no. 4 SUPPL., pp. 1093-1099. https://doi.org/10.1016/j.fertnstert.2004.12.013
Kim, Khae Hawn ; Joo, Kwan Joong ; Park, Heung Jae ; Kwon, Chil Hun ; Jang, Mi-Hyeon D ; Kim, Chang Ju. / Nicotine induces apoptosis in TM3 mouse Leydig cells. In: Fertility and Sterility. 2005 ; Vol. 83, No. 4 SUPPL. pp. 1093-1099.
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