Nicotinate adenine dinucleotide phosphate (NAADP) triggers a specific calcium release system in sea urchin eggs

Eduardo Nunes Chini, K. W. Beers, T. P. Dousa

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Transient fluxes of intracellular ionized calcium (Ca2+) from intracellular stores are integral components of regulatory signaling pathways operating in numerous biological regulations, including in early stages of egg fertilization. Therefore, we explored whether NADP, which is rapidly generated by phosphorylation of NAD upon fertilization may, directly or indirectly, exert a regulatory role as a trigger of Ca2+ release from intracellular stores in sea urchin eggs. NADP had no effect, but we found that the deamidated derivative of NADP, nicotinate adenine dinucleotide phosphate (β-NAADP), is a potent and specific stimulus (ED50 16 nM) for Ca2+ release in sea urchin egg homogenates. NAADP triggers the Ca2+ release via a mechanism which is distinct from the well-known Ca2+ release systems triggered either by inositol-1,4,5-triphosphate (IP3) or by cyclic adenosine diphospho-ribose (cADPR). The NAADP-induced release of Ca2+ is not blocked by heparin, and antagonist of IP3, or by procaine or ruthenium red, antagonists of cADPR. However, it is selectively blocked by thionicotinamide. NADP which does not inhibit the actions of IP3 or cADPR. NAADP produced by heating of NADP in alkaline (pH = 12) medium or synthetized enzymatically by nicotinic acid-NADP reaction catalyzed by NAD glycohydrolase have identical properties. The results presented herein thus describe a novel endocellular Ca2+-releasing system controlled by NAADP as a specific stimulus. The NAADP-controlled Ca2+ release system may be an integral component of multiple intracellular regulations occurring in fertilized sea urchin eggs, which are mediated by intracellular Ca2+ release, and may also have similar role(s) in other tissues.

Original languageEnglish (US)
Pages (from-to)3216-3223
Number of pages8
JournalJournal of Biological Chemistry
Volume270
Issue number7
DOIs
StatePublished - 1995

Fingerprint

Sea Urchins
NADP
Eggs
Calcium
Ribose
Adenosine
Fertilization
Ovum
Heparin Antagonists
NAD+ Nucleosidase
Ruthenium Red
Procaine
Phosphorylation
Inositol 1,4,5-Trisphosphate
Niacin
NAD
Heating
NAADP
Tissue
Fluxes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nicotinate adenine dinucleotide phosphate (NAADP) triggers a specific calcium release system in sea urchin eggs. / Chini, Eduardo Nunes; Beers, K. W.; Dousa, T. P.

In: Journal of Biological Chemistry, Vol. 270, No. 7, 1995, p. 3216-3223.

Research output: Contribution to journalArticle

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