Next-generation sequencing to identify genetic causes of cardiomyopathies

Nadine Norton, Duanxiang Li, Ray E. Hershberger

Research output: Contribution to journalReview article

27 Scopus citations

Abstract

Purpose of Review: This review examines the application of next-generation sequencing (NGS) technologies in the identification of the causation of nonsyndromic genetic cardiomyopathies. Recent Findings: NGS sequencing of the entire genetic coding sequence (the exome) has successfully identified five novel genes and causative variants for cardiomyopathies without previously known cause within the last 12 months. Continual rapidly decreasing costs of NGS will shortly allow cost-effective sequencing of the entire genomes of affected individuals and their relatives to include noncoding and regulatory variant discovery and epigenetic profiling. Despite this rapid technological progress with sequencing, analysis of these large data sets remains challenging, particularly for assigning causality to novel rare variants identified in DNA samples from patients with cardiomyopathy. Summary: NGS technologies are rapidly moving to identify novel rare variants in patients with cardiomyopathy, but assigning pathogenicity to these novel variants remains challenging.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalCurrent opinion in cardiology
Volume27
Issue number3
DOIs
StatePublished - May 1 2012

Keywords

  • cardiomyopathy
  • exome
  • genetics
  • genomics
  • next-generation sequencing

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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