New TLR7 agonists with improved humoral and cellular immune responses

Katherine C. Upchurch, José R. Boquín, Wenjie Yin, Yaming Xue, HyeMee Joo, Robert R. Kane, SangKon Oh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Toll-like receptor 7 (TLR7) agonists are of interest as vaccine adjuvants and cancer therapeutics. Therefore, development of new TLR7 agonists that can efficiently promote host immune responses without evoking side effects is of great importance. Here, we describe two new compounds, J4 and F4, which elicit intracellular signaling exclusively via TLR7. Interestingly, both J4 and F4 induced less cytokine secretion (IL-1β, IL-6, IL-10, IL-12p40, TNFα, and IL-12p70) from myeloid dendritic cells (mDCs) and monocytes than CL075 and R848; however, they all generated similar levels of phenotype maturation of antigen presenting cells (APCs), including plasmacytoid DCs. We further found that J4- and F4-induced APC activation was largely dependent on the activation of NF-κB and p38. Lastly, J4 and F4 could efficiently promote B cell proliferation and plasmablast differentiation as well as antigen-specific CD8+ T cell responses in human in vitro. Therefore, these new TLR7 agonists could be employed to facilitate the development of new therapeutics and vaccine adjuvants against cancers and microbial infections.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalImmunology Letters
Volume168
Issue number1
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Toll-Like Receptor 7
Humoral Immunity
Cellular Immunity
Antigen-Presenting Cells
resiquimod
Interleukin-12 Subunit p40
CD8 Antigens
Cancer Vaccines
Myeloid Cells
Interleukin-1
Interleukin-10
Dendritic Cells
Monocytes
Interleukin-6
B-Lymphocytes
Vaccines
Cell Proliferation
Cytokines
T-Lymphocytes
Phenotype

Keywords

  • Adjuvant
  • Dendritic Cell
  • TLR7
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

New TLR7 agonists with improved humoral and cellular immune responses. / Upchurch, Katherine C.; Boquín, José R.; Yin, Wenjie; Xue, Yaming; Joo, HyeMee; Kane, Robert R.; Oh, SangKon.

In: Immunology Letters, Vol. 168, No. 1, 01.11.2015, p. 89-97.

Research output: Contribution to journalArticle

Upchurch, Katherine C. ; Boquín, José R. ; Yin, Wenjie ; Xue, Yaming ; Joo, HyeMee ; Kane, Robert R. ; Oh, SangKon. / New TLR7 agonists with improved humoral and cellular immune responses. In: Immunology Letters. 2015 ; Vol. 168, No. 1. pp. 89-97.
@article{7bfabecbd07c4a229b7beb67a872ceb6,
title = "New TLR7 agonists with improved humoral and cellular immune responses",
abstract = "Toll-like receptor 7 (TLR7) agonists are of interest as vaccine adjuvants and cancer therapeutics. Therefore, development of new TLR7 agonists that can efficiently promote host immune responses without evoking side effects is of great importance. Here, we describe two new compounds, J4 and F4, which elicit intracellular signaling exclusively via TLR7. Interestingly, both J4 and F4 induced less cytokine secretion (IL-1β, IL-6, IL-10, IL-12p40, TNFα, and IL-12p70) from myeloid dendritic cells (mDCs) and monocytes than CL075 and R848; however, they all generated similar levels of phenotype maturation of antigen presenting cells (APCs), including plasmacytoid DCs. We further found that J4- and F4-induced APC activation was largely dependent on the activation of NF-κB and p38. Lastly, J4 and F4 could efficiently promote B cell proliferation and plasmablast differentiation as well as antigen-specific CD8+ T cell responses in human in vitro. Therefore, these new TLR7 agonists could be employed to facilitate the development of new therapeutics and vaccine adjuvants against cancers and microbial infections.",
keywords = "Adjuvant, Dendritic Cell, TLR7, Vaccine",
author = "Upchurch, {Katherine C.} and Boqu{\'i}n, {Jos{\'e} R.} and Wenjie Yin and Yaming Xue and HyeMee Joo and Kane, {Robert R.} and SangKon Oh",
year = "2015",
month = "11",
day = "1",
doi = "10.1016/j.imlet.2015.09.007",
language = "English (US)",
volume = "168",
pages = "89--97",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - New TLR7 agonists with improved humoral and cellular immune responses

AU - Upchurch, Katherine C.

AU - Boquín, José R.

AU - Yin, Wenjie

AU - Xue, Yaming

AU - Joo, HyeMee

AU - Kane, Robert R.

AU - Oh, SangKon

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Toll-like receptor 7 (TLR7) agonists are of interest as vaccine adjuvants and cancer therapeutics. Therefore, development of new TLR7 agonists that can efficiently promote host immune responses without evoking side effects is of great importance. Here, we describe two new compounds, J4 and F4, which elicit intracellular signaling exclusively via TLR7. Interestingly, both J4 and F4 induced less cytokine secretion (IL-1β, IL-6, IL-10, IL-12p40, TNFα, and IL-12p70) from myeloid dendritic cells (mDCs) and monocytes than CL075 and R848; however, they all generated similar levels of phenotype maturation of antigen presenting cells (APCs), including plasmacytoid DCs. We further found that J4- and F4-induced APC activation was largely dependent on the activation of NF-κB and p38. Lastly, J4 and F4 could efficiently promote B cell proliferation and plasmablast differentiation as well as antigen-specific CD8+ T cell responses in human in vitro. Therefore, these new TLR7 agonists could be employed to facilitate the development of new therapeutics and vaccine adjuvants against cancers and microbial infections.

AB - Toll-like receptor 7 (TLR7) agonists are of interest as vaccine adjuvants and cancer therapeutics. Therefore, development of new TLR7 agonists that can efficiently promote host immune responses without evoking side effects is of great importance. Here, we describe two new compounds, J4 and F4, which elicit intracellular signaling exclusively via TLR7. Interestingly, both J4 and F4 induced less cytokine secretion (IL-1β, IL-6, IL-10, IL-12p40, TNFα, and IL-12p70) from myeloid dendritic cells (mDCs) and monocytes than CL075 and R848; however, they all generated similar levels of phenotype maturation of antigen presenting cells (APCs), including plasmacytoid DCs. We further found that J4- and F4-induced APC activation was largely dependent on the activation of NF-κB and p38. Lastly, J4 and F4 could efficiently promote B cell proliferation and plasmablast differentiation as well as antigen-specific CD8+ T cell responses in human in vitro. Therefore, these new TLR7 agonists could be employed to facilitate the development of new therapeutics and vaccine adjuvants against cancers and microbial infections.

KW - Adjuvant

KW - Dendritic Cell

KW - TLR7

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=84942746341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942746341&partnerID=8YFLogxK

U2 - 10.1016/j.imlet.2015.09.007

DO - 10.1016/j.imlet.2015.09.007

M3 - Article

VL - 168

SP - 89

EP - 97

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 1

ER -