New mammalian chloride channel identified by expression cloning

Markus Paulmichl, Yi Li, Kevin Wickman, Michael John Ackerman, Ernest Peralta, David Clapham

Research output: Contribution to journalArticle

296 Citations (Scopus)

Abstract

ION channels selectively permeable to chloride ions regulate cell functions as diverse as excitability and control of cell volume1-5. Using expression cloning techniques, a complementary DNA from an epithelial cell line has been isolated, sequenced and its putative structure examined by site-directed mutagenesis. This cDNA, encoding a 235-amino-acid protein, gave rise to a chloride-selective outward current when expressed in Xenopus oocytes. The expressed, outwardly rectifying chloride current was calcium-insensitive and was blocked by nucleotides applied to the cell surface. Mutation of a putative nucleotide-binding site resulted in loss of nucleotide block but incurred dependence on extracellular calcium concentration. The unusual sequence of this putative channel protein suggests a new class of ion channels not related to other previously cloned chloride channels6-11.

Original languageEnglish (US)
Pages (from-to)238-241
Number of pages4
JournalNature
Volume356
Issue number6366
StatePublished - Mar 19 1992

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Chloride Channels
Organism Cloning
Chlorides
Nucleotides
Complementary DNA
Calcium
Xenopus
Site-Directed Mutagenesis
Ion Channels
Oocytes
Proteins
Epithelial Cells
Binding Sites
Ions
Amino Acids
Cell Line
Mutation

ASJC Scopus subject areas

  • General

Cite this

Paulmichl, M., Li, Y., Wickman, K., Ackerman, M. J., Peralta, E., & Clapham, D. (1992). New mammalian chloride channel identified by expression cloning. Nature, 356(6366), 238-241.

New mammalian chloride channel identified by expression cloning. / Paulmichl, Markus; Li, Yi; Wickman, Kevin; Ackerman, Michael John; Peralta, Ernest; Clapham, David.

In: Nature, Vol. 356, No. 6366, 19.03.1992, p. 238-241.

Research output: Contribution to journalArticle

Paulmichl, M, Li, Y, Wickman, K, Ackerman, MJ, Peralta, E & Clapham, D 1992, 'New mammalian chloride channel identified by expression cloning', Nature, vol. 356, no. 6366, pp. 238-241.
Paulmichl M, Li Y, Wickman K, Ackerman MJ, Peralta E, Clapham D. New mammalian chloride channel identified by expression cloning. Nature. 1992 Mar 19;356(6366):238-241.
Paulmichl, Markus ; Li, Yi ; Wickman, Kevin ; Ackerman, Michael John ; Peralta, Ernest ; Clapham, David. / New mammalian chloride channel identified by expression cloning. In: Nature. 1992 ; Vol. 356, No. 6366. pp. 238-241.
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