Neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1, and carbohydrate antigen 19-9 in pancreatic juice: Pathobiologic implications in diagnosing benign and malignant disease of the pancreas

Sukhwinder Kaur, Michael J. Baine, Sushovan Guha, Nobuo Ochi, Subhankar Chakraborty, Kavita Mallya, Colleen Thomas, Juliana Crook, Michael B. Wallace, Timothy A. Woodward, Maneesh Jain, Shailender Singh, Aaron R. Sasson, Verna Skinner, Massimo Raimondo, Surinder K. Batra

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

OBJECTIVE: Pancreatic diseases pose significant diagnostic challenge as signs and symptoms often overlap. We investigated the potential of pancreatic juice neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1 (MIC-1), and carbohydrate antigen 19-9 (CA19-9) to aid in the diagnosis of patients with symptoms suggestive of pancreatic diseases. METHODS: A total of 105 chronic pancreatitis (CP), pancreatic cancer (PC), and nonpancreatic nonhealthy (patients with symptoms mimicking pancreatic disease but found to be free of any pancreatic disease) patients underwent endoscopic pancreatic juice collection after secretin stimulation. Neutrophil gelatinase-associated lipocalin and MIC-1 levels were measured by enzyme-linked immunosorbent assay, whereas CA19-9 was measured by radioimmunoassay. RESULTS: Neutrophil gelatinase-associated lipocalin, MIC-1, and CA19-9 were significantly elevated in the pancreatic juice of patients with CP and patients with PC as compared with nonpancreatic nonhealthy controls (P ≤ 0.034). Neutrophil gelatinase-associated lipocalin seemed most promising in differentiating diseased versus nondiseased pancreata (areas under the curve, 0.88-0.91), whereas MIC-1 was found to be higher in patients with PC than in patients with CP (P = 0.043). Interestingly, MIC-1 levels in diabetic patients with PC were higher than in nondiabetic patients with PC (P = 0.030) and diabetic patients with CP (P = 0.087). Carbohydrate antigen 19-9 showed the least ability to distinguish patient groups (areas under the curve, 0.61-0.76). CONCLUSIONS: Pancreatic juice neutrophil gelatinase-associated lipocalin shows potential utility in establishing pancreatic etiology in the context of nonspecific symptoms, whereas MIC-1 may aid in differentiating PC from CP.

Original languageEnglish (US)
Pages (from-to)494-501
Number of pages8
JournalPancreas
Volume42
Issue number3
DOIs
StatePublished - Apr 2013

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Growth Differentiation Factor 15
Pancreatic Juice
Pancreas
Carbohydrates
Antigens
Pancreatic Neoplasms
Chronic Pancreatitis
Pancreatic Diseases
Area Under Curve
Lipocalin-2
Secretin
Signs and Symptoms
Radioimmunoassay

Keywords

  • CA19-9
  • MIC-1
  • NGAL
  • Pancreatic disease
  • Pancreatic juice

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1, and carbohydrate antigen 19-9 in pancreatic juice : Pathobiologic implications in diagnosing benign and malignant disease of the pancreas. / Kaur, Sukhwinder; Baine, Michael J.; Guha, Sushovan; Ochi, Nobuo; Chakraborty, Subhankar; Mallya, Kavita; Thomas, Colleen; Crook, Juliana; Wallace, Michael B.; Woodward, Timothy A.; Jain, Maneesh; Singh, Shailender; Sasson, Aaron R.; Skinner, Verna; Raimondo, Massimo; Batra, Surinder K.

In: Pancreas, Vol. 42, No. 3, 04.2013, p. 494-501.

Research output: Contribution to journalArticle

Kaur, S, Baine, MJ, Guha, S, Ochi, N, Chakraborty, S, Mallya, K, Thomas, C, Crook, J, Wallace, MB, Woodward, TA, Jain, M, Singh, S, Sasson, AR, Skinner, V, Raimondo, M & Batra, SK 2013, 'Neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1, and carbohydrate antigen 19-9 in pancreatic juice: Pathobiologic implications in diagnosing benign and malignant disease of the pancreas', Pancreas, vol. 42, no. 3, pp. 494-501. https://doi.org/10.1097/MPA.0b013e31826a8597
Kaur, Sukhwinder ; Baine, Michael J. ; Guha, Sushovan ; Ochi, Nobuo ; Chakraborty, Subhankar ; Mallya, Kavita ; Thomas, Colleen ; Crook, Juliana ; Wallace, Michael B. ; Woodward, Timothy A. ; Jain, Maneesh ; Singh, Shailender ; Sasson, Aaron R. ; Skinner, Verna ; Raimondo, Massimo ; Batra, Surinder K. / Neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1, and carbohydrate antigen 19-9 in pancreatic juice : Pathobiologic implications in diagnosing benign and malignant disease of the pancreas. In: Pancreas. 2013 ; Vol. 42, No. 3. pp. 494-501.
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abstract = "OBJECTIVE: Pancreatic diseases pose significant diagnostic challenge as signs and symptoms often overlap. We investigated the potential of pancreatic juice neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1 (MIC-1), and carbohydrate antigen 19-9 (CA19-9) to aid in the diagnosis of patients with symptoms suggestive of pancreatic diseases. METHODS: A total of 105 chronic pancreatitis (CP), pancreatic cancer (PC), and nonpancreatic nonhealthy (patients with symptoms mimicking pancreatic disease but found to be free of any pancreatic disease) patients underwent endoscopic pancreatic juice collection after secretin stimulation. Neutrophil gelatinase-associated lipocalin and MIC-1 levels were measured by enzyme-linked immunosorbent assay, whereas CA19-9 was measured by radioimmunoassay. RESULTS: Neutrophil gelatinase-associated lipocalin, MIC-1, and CA19-9 were significantly elevated in the pancreatic juice of patients with CP and patients with PC as compared with nonpancreatic nonhealthy controls (P ≤ 0.034). Neutrophil gelatinase-associated lipocalin seemed most promising in differentiating diseased versus nondiseased pancreata (areas under the curve, 0.88-0.91), whereas MIC-1 was found to be higher in patients with PC than in patients with CP (P = 0.043). Interestingly, MIC-1 levels in diabetic patients with PC were higher than in nondiabetic patients with PC (P = 0.030) and diabetic patients with CP (P = 0.087). Carbohydrate antigen 19-9 showed the least ability to distinguish patient groups (areas under the curve, 0.61-0.76). CONCLUSIONS: Pancreatic juice neutrophil gelatinase-associated lipocalin shows potential utility in establishing pancreatic etiology in the context of nonspecific symptoms, whereas MIC-1 may aid in differentiating PC from CP.",
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T2 - Pathobiologic implications in diagnosing benign and malignant disease of the pancreas

AU - Kaur, Sukhwinder

AU - Baine, Michael J.

AU - Guha, Sushovan

AU - Ochi, Nobuo

AU - Chakraborty, Subhankar

AU - Mallya, Kavita

AU - Thomas, Colleen

AU - Crook, Juliana

AU - Wallace, Michael B.

AU - Woodward, Timothy A.

AU - Jain, Maneesh

AU - Singh, Shailender

AU - Sasson, Aaron R.

AU - Skinner, Verna

AU - Raimondo, Massimo

AU - Batra, Surinder K.

PY - 2013/4

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N2 - OBJECTIVE: Pancreatic diseases pose significant diagnostic challenge as signs and symptoms often overlap. We investigated the potential of pancreatic juice neutrophil gelatinase-associated lipocalin, macrophage inhibitory cytokine 1 (MIC-1), and carbohydrate antigen 19-9 (CA19-9) to aid in the diagnosis of patients with symptoms suggestive of pancreatic diseases. METHODS: A total of 105 chronic pancreatitis (CP), pancreatic cancer (PC), and nonpancreatic nonhealthy (patients with symptoms mimicking pancreatic disease but found to be free of any pancreatic disease) patients underwent endoscopic pancreatic juice collection after secretin stimulation. Neutrophil gelatinase-associated lipocalin and MIC-1 levels were measured by enzyme-linked immunosorbent assay, whereas CA19-9 was measured by radioimmunoassay. RESULTS: Neutrophil gelatinase-associated lipocalin, MIC-1, and CA19-9 were significantly elevated in the pancreatic juice of patients with CP and patients with PC as compared with nonpancreatic nonhealthy controls (P ≤ 0.034). Neutrophil gelatinase-associated lipocalin seemed most promising in differentiating diseased versus nondiseased pancreata (areas under the curve, 0.88-0.91), whereas MIC-1 was found to be higher in patients with PC than in patients with CP (P = 0.043). Interestingly, MIC-1 levels in diabetic patients with PC were higher than in nondiabetic patients with PC (P = 0.030) and diabetic patients with CP (P = 0.087). Carbohydrate antigen 19-9 showed the least ability to distinguish patient groups (areas under the curve, 0.61-0.76). CONCLUSIONS: Pancreatic juice neutrophil gelatinase-associated lipocalin shows potential utility in establishing pancreatic etiology in the context of nonspecific symptoms, whereas MIC-1 may aid in differentiating PC from CP.

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KW - CA19-9

KW - MIC-1

KW - NGAL

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KW - Pancreatic juice

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