Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A)

Isobel A. Scarisbrick; Kunihiko Asakura; Moses Rodriguez

doi:10.1016/S0165-3806(00)00077-8

Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A)

Isobel A. Scarisbrick, Kunihiko Asakura, Moses Rodriguez

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology. (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	87-90
Number of pages	4
Journal	Developmental Brain Research
Volume	123
Issue number	1
DOIs	https://doi.org/10.1016/S0165-3806(00)00077-8
State	Published - Sep 30 2000

Keywords

CNS
Development
Glia
Growth factor
Rat

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/S0165-3806(00)00077-8

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title = "Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A)",

abstract = "In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology. (C) 2000 Elsevier Science B.V.",

keywords = "CNS, Development, Glia, Growth factor, Rat",

author = "Scarisbrick, {Isobel A.} and Kunihiko Asakura and Moses Rodriguez",

note = "Funding Information: This research was supported by Grant PP0725 from the National Multiple Sclerosis Society to I.A.S., by the Mayo Foundation and by Training Grant T32 HD 07447-8, directed by Dr. J.R. Basford, Department of Physical Medicine and Rehabilitation, Mayo Clinic and Foundation. Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.",

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doi = "10.1016/S0165-3806(00)00077-8",

language = "English (US)",

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pages = "87--90",

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AU - Scarisbrick, Isobel A.

AU - Asakura, Kunihiko

AU - Rodriguez, Moses

N1 - Funding Information: This research was supported by Grant PP0725 from the National Multiple Sclerosis Society to I.A.S., by the Mayo Foundation and by Training Grant T32 HD 07447-8, directed by Dr. J.R. Basford, Department of Physical Medicine and Rehabilitation, Mayo Clinic and Foundation. Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

PY - 2000/9/30

Y1 - 2000/9/30

N2 - In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology. (C) 2000 Elsevier Science B.V.

AB - In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology. (C) 2000 Elsevier Science B.V.

KW - CNS

KW - Development

KW - Glia

KW - Growth factor

KW - Rat

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Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A)

Abstract

Keywords

ASJC Scopus subject areas

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