Neurotransmitter synthesis, storage and release by aggregating cell cultures of rat brain

Paul Honegger, Elliott Richelson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Rotation-mediated aggregating cell cultures of mechanically dissociated fetal (15-16 days gestation) rat brains between 25 and 35 days in vitro were examined for their ability to synthesize neurotransmitters and putative neurotransmitters from radioactively labeled precursors added to the culture medium. Cultures derived from whole brain synthesized [3H]acetylcholine from [3H]choline, [3H]γ-aminobutyric acid from l-[3H]glutamic acid, [3H]dopamine from l-[3H]tyrosine, [3H]dopamine and [3H]norepinephrine from l-[3H]dihydroxyphenylalanine, and [3H]serotonin from l-[3H]tryptophan. Veratridine increased and tetrodotoxin decreased the rate of [3H]-dopamine synthesized by aggregates derived from midbrain plus hindbrain. In chase experiments in which aggregates were incubated for 4 h with radioactively labeled precursors and then for 4 h with non-radioactively labeled precursors, addition of veratridine (50 μM) during the second 4 h incubation significantly decreased the amounts of radioactively labeled acetylcholine, l-glutamic acid, dopamine and serotonin recovered from aggregates. Tetrodotoxin (5 μM) present during the chase significantly increased the amounts of [3H]acetylcholine and [3H]dopamine recovered from the aggregates. In addition, reserpine (4 μM) markedly depleted [3H]dopamine from aggregates in these experiments. These results indicate that these cultured cells synthesized neurotransmitters and in addition suggest that some of these compounds are stored by and released from electrically active cells within the aggregates.

Original languageEnglish (US)
Pages (from-to)89-101
Number of pages13
JournalBrain Research
Volume162
Issue number1
DOIs
StatePublished - Feb 16 1979

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Neurotransmitter Agents
Dopamine
Cell Culture Techniques
Brain
Veratridine
Acetylcholine
Tetrodotoxin
Glutamic Acid
Serotonin
Aminobutyrates
Rhombencephalon
Dihydroxyphenylalanine
Reserpine
Mesencephalon
Choline
Tryptophan
Tyrosine
Culture Media
Cultured Cells
Norepinephrine

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neurotransmitter synthesis, storage and release by aggregating cell cultures of rat brain. / Honegger, Paul; Richelson, Elliott.

In: Brain Research, Vol. 162, No. 1, 16.02.1979, p. 89-101.

Research output: Contribution to journalArticle

Honegger, Paul ; Richelson, Elliott. / Neurotransmitter synthesis, storage and release by aggregating cell cultures of rat brain. In: Brain Research. 1979 ; Vol. 162, No. 1. pp. 89-101.
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