Neurotensin stimulates inositol phospholipid metabolism and calcium mobilization in murine neuroblastoma clone N1E-115

R. M. Snider, C. Forray, M. Pfenning, E. Richelson

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Abstract

Murine neuroblastoma cells (clone N1E-115) possess neurotensin receptors that mediate cyclic GMP synthesis. Because of the hypothesized relationship between phospholipid metabolism, intracellular Ca 2+, and cyclic GMP synthesis, we determined with these cells the effects of neurotensin on 32P labeling of phospholipids, release of inositol phosphates, and intracellular Ca 2+ (as determined with the use of Quin-2, a fluorescent probe sensitive to free Ca 2+ levels). Neurotensin stimulated incorporation of 32P into phospholipids, especially phosphatidylinositol and phosphatidate. Neurotensin also stimulated the release of [ 3H]inositol phosphates with an EC 50 of about 1 nM. Mean basal Ca 2+ concentration in these cells was 134 nM and this level was increased in a rapid and dose-dependent manner by neurotensin, with an EC 50 of 4 nM. Since the EC 50 for neurotensin in stimulating cyclic GMP synthesis is 1.5 nM and the K(D) for binding of [ 3H]neurotensin at 0°C is 11 nM, all these different effects appear to be shared proximal consequences of neurotensin receptor activation.

Original languageEnglish (US)
Pages (from-to)1214-1218
Number of pages5
JournalJournal of Neurochemistry
Volume47
Issue number4
StatePublished - 1986

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Neurotensin
Phosphatidylinositols
Neuroblastoma
Metabolism
Clone Cells
Calcium
Cyclic GMP
Neurotensin Receptors
Phospholipids
Inositol Phosphates
Clone cells
Fluorescent Dyes
Labeling
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neurotensin stimulates inositol phospholipid metabolism and calcium mobilization in murine neuroblastoma clone N1E-115. / Snider, R. M.; Forray, C.; Pfenning, M.; Richelson, E.

In: Journal of Neurochemistry, Vol. 47, No. 4, 1986, p. 1214-1218.

Research output: Contribution to journalArticle

Snider, R. M. ; Forray, C. ; Pfenning, M. ; Richelson, E. / Neurotensin stimulates inositol phospholipid metabolism and calcium mobilization in murine neuroblastoma clone N1E-115. In: Journal of Neurochemistry. 1986 ; Vol. 47, No. 4. pp. 1214-1218.
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