Neurotensin Stimulates Inositol Phospholipid Metabolism and Calcium Mobilization in Murine Neuroblastoma Clone N1E‐115

R. M. Snider, C. Forray, M. Pfenning, E. Richelson

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Abstract Murine neuroblastoma cells (clone N1E‐115) possess neurotensin receptors that mediate cyclic GMP synthesis. Because of the hypothesized relationship between phospholipid metabolism, intracellular Ca2+, and cyclic GMP synthesis, we determined with these cells the effects of neurotensin on 32P labeling of phospholipids, release of inositol phosphates, and intracellular Ca2+ (as determined with the use of Quin‐2, a fluorescent probe sensitive to free Ca2+ levels). Neurotensin stimulated incorporation of 32P into phospholipids, especially phosphatidylinositol and phosphatidate. Neurotensin also stimulated the release of [3H]‐inositol phosphates with an EC50 of about 1 nM. Mean basal Ca2+ concentration in these cells was 134 nM and this level was increased in a rapid and dose‐dependent manner by neurotensin, with an EC50 of 4 nM. Since the EC50 for neurotensin in stimulating cyclic GMP synthesis is 1.5 nM and the KD for binding of [3H]neurotensin at 0° is 11 nM, all these different effects appear to be shared proximal consequences of neurotensin receptor activation.

Original languageEnglish (US)
Pages (from-to)1214-1218
Number of pages5
JournalJournal of neurochemistry
Volume47
Issue number4
DOIs
StatePublished - Oct 1986

Keywords

  • Cyclic GMP
  • Inositol phosphate
  • N1E‐115
  • Neurotensin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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