Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor

Misa Yamada; Mitsuhiko Yamada; Michael A. Watson; Elliott Richelson

doi:10.1016/0922-4106(93)90064-G

Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor

Misa Yamada, Mitsuhiko Yamada, Michael A. Watson, Elliott Richelson

Neuroscience

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC₅₀ = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca²⁺-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca²⁺ concentration from polyphosphoinositide hydrolysis.

Original language	English (US)
Pages (from-to)	99-101
Number of pages	3
Journal	European Journal of Pharmacology: Molecular Pharmacology
Volume	244
Issue number	1
DOIs	https://doi.org/10.1016/0922-4106(93)90064-G
State	Published - Jan 4 1993

Keywords

AMP formation
Neurotensin
Promiscuous coupling
Transfection

ASJC Scopus subject areas

Pharmacology

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10.1016/0922-4106(93)90064-G

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title = "Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor",

abstract = "Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC50 = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca2+-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca2+ concentration from polyphosphoinositide hydrolysis.",

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AU - Yamada, Misa

AU - Yamada, Mitsuhiko

AU - Watson, Michael A.

AU - Richelson, Elliott

PY - 1993/1/4

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AB - Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC50 = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca2+-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca2+ concentration from polyphosphoinositide hydrolysis.

KW - AMP formation

KW - Neurotensin

KW - Promiscuous coupling

KW - Transfection

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Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor

Abstract

Keywords

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