Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor

Misa Yamada, Mitsuhiko Yamada, Michael A. Watson, Elliott Richelson

Research output: Contribution to journalArticle

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Abstract

Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC50 = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca2+-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca2+ concentration from polyphosphoinositide hydrolysis.

Original languageEnglish (US)
Pages (from-to)99-101
Number of pages3
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume244
Issue number1
DOIs
StatePublished - Jan 4 1993

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Keywords

  • AMP formation
  • Neurotensin
  • Promiscuous coupling
  • Transfection

ASJC Scopus subject areas

  • Pharmacology

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