TY - JOUR
T1 - Neurosphere culture and human organotypic model to evaluate brain tumor stem cells.
AU - Guerrero-Cázares, Hugo
AU - Chaichana, Kaisorn L.
AU - Quiñones-Hinojosa, Alfredo
PY - 2009
Y1 - 2009
N2 - The brain tumor stem cell (BTSC) hypothesis is based on the premise that there is a subpopulation of cells within tumors with tumorigenic and pluripotent properties. BTSC are believed to be responsible for both the initiation of brain tumors and their resistance to current therapeutic modalities. This new paradigm stresses the need for adequate techniques to culture and characterize this special population of cells. Furthermore, the use of different cell migration assays offers the possibility to evaluate the processes involved in glioma metastasis. In this chapter, we summarize a method to culture, analyze the cellular characteristics, and study the invasion of BTSCs using a neurosphere assay, cryostat sectioning, and human organotypic brain cortex migration assay, respectively.
AB - The brain tumor stem cell (BTSC) hypothesis is based on the premise that there is a subpopulation of cells within tumors with tumorigenic and pluripotent properties. BTSC are believed to be responsible for both the initiation of brain tumors and their resistance to current therapeutic modalities. This new paradigm stresses the need for adequate techniques to culture and characterize this special population of cells. Furthermore, the use of different cell migration assays offers the possibility to evaluate the processes involved in glioma metastasis. In this chapter, we summarize a method to culture, analyze the cellular characteristics, and study the invasion of BTSCs using a neurosphere assay, cryostat sectioning, and human organotypic brain cortex migration assay, respectively.
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U2 - 10.1007/978-1-59745-280-9_6
DO - 10.1007/978-1-59745-280-9_6
M3 - Article
C2 - 19582422
AN - SCOPUS:70449707805
SN - 1064-3745
VL - 568
SP - 73
EP - 83
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -