Neurosphere assays: Growth factors and hormone differences in tumor and nontumor studies

Kaisorn Chaichana, Grettel Zamora-Berridi, Joaquin Camara-Quintana, Alfredo Quinones-Hinojosa

Research output: Contribution to journalReview article

65 Citations (Scopus)

Abstract

The "no new neuron" dogma that the brain is quiescent throughout adult life has been challenged by the discovery of cells with stem cell-like qualities of self-renewal and multipotency in the subventricular zone of the lateral ventricles and the dentate gyrus of the hippocampus in adults. This self-renewing capacity also makes these neural stem cells a possible source of brain tumors, which was supported by the discovery of self-sustaining brain tumor stem-like cells in cancers such as glioblastoma multiforme. Neurosphere assays are the standard for studying these stem-like cells in both normal and cancer tissues. Despite the importance of these assays, there is no standardized protocol to allow for a comparison of results because several studies use different growth factors and hormones at different concentrations. The primary objective of this study is to review the literature for both nontumor and tumor studies to assess their respective neurosphere assay components. We found significant variation in assay components, namely hormones and growth factors, as well as their respective concentrations. This illustrates the need for a standardized protocol to allow proper comparison among studies and a better assessment of the effects of different factors.

Original languageEnglish (US)
Pages (from-to)2851-2857
Number of pages7
JournalStem Cells
Volume24
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Growth Hormone
Intercellular Signaling Peptides and Proteins
Lateral Ventricles
Brain Neoplasms
Stem Cells
Neoplasms
Parahippocampal Gyrus
Neural Stem Cells
Neoplastic Stem Cells
Dentate Gyrus
Glioblastoma
Hormones
Neurons
Brain

Keywords

  • Brain tumor stem cells
  • Neural stem cells
  • Neurosphere assay

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Neurosphere assays : Growth factors and hormone differences in tumor and nontumor studies. / Chaichana, Kaisorn; Zamora-Berridi, Grettel; Camara-Quintana, Joaquin; Quinones-Hinojosa, Alfredo.

In: Stem Cells, Vol. 24, No. 12, 12.2006, p. 2851-2857.

Research output: Contribution to journalReview article

Chaichana, Kaisorn ; Zamora-Berridi, Grettel ; Camara-Quintana, Joaquin ; Quinones-Hinojosa, Alfredo. / Neurosphere assays : Growth factors and hormone differences in tumor and nontumor studies. In: Stem Cells. 2006 ; Vol. 24, No. 12. pp. 2851-2857.
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