Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices

K. M. Schak, S. P. Scordilis, G. A. Ferreyra, M. E. Harrington

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalBiological Rhythm Research
Volume32
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

neuropeptide Y
Suprachiasmatic Nucleus
Neuropeptide Y
protein kinase C
hamsters
Cricetinae
Protein Kinase C
brain
protein
Brain
Circadian Clocks
cerebrospinal fluid
circadian rhythm
Cerebrospinal Fluid
fluid

Keywords

  • Circadian
  • Neuropeptide Y
  • Protein kinase C assay
  • Rhythm
  • Suprachiasmatic

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Physiology

Cite this

Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices. / Schak, K. M.; Scordilis, S. P.; Ferreyra, G. A.; Harrington, M. E.

In: Biological Rhythm Research, Vol. 32, No. 2, 2001, p. 201-206.

Research output: Contribution to journalArticle

Schak, K. M. ; Scordilis, S. P. ; Ferreyra, G. A. ; Harrington, M. E. / Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices. In: Biological Rhythm Research. 2001 ; Vol. 32, No. 2. pp. 201-206.
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