Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices

K. M. Schak; S. P. Scordilis; G. A. Ferreyra; M. E. Harrington

doi:10.1076/brhm.32.2.201.1358

Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices

K. M. Schak, S. P. Scordilis, G. A. Ferreyra, M. E. Harrington

Psychiatry and Psychology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.

Original language	English (US)
Pages (from-to)	201-206
Number of pages	6
Journal	Biological Rhythm Research
Volume	32
Issue number	2
DOIs	https://doi.org/10.1076/brhm.32.2.201.1358
State	Published - 2001

Keywords

Circadian
Neuropeptide Y
Protein kinase C assay
Rhythm
Suprachiasmatic

ASJC Scopus subject areas

Physiology (medical)
Ecology, Evolution, Behavior and Systematics
Physiology

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10.1076/brhm.32.2.201.1358

Cite this

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title = "Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices",

abstract = "The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.",

keywords = "Circadian, Neuropeptide Y, Protein kinase C assay, Rhythm, Suprachiasmatic",

author = "Schak, {K. M.} and Scordilis, {S. P.} and Ferreyra, {G. A.} and Harrington, {M. E.}",

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AU - Schak, K. M.

AU - Scordilis, S. P.

AU - Ferreyra, G. A.

AU - Harrington, M. E.

N1 - Funding Information: This study was supported by NIH grant NS26496 (M.E.H.).

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N2 - The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.

AB - The mammalian circadian clock in the suprachiasmatic nuclei (SCN) can be phase-shifted by neuropeptide Y applied in the subjective day. Previous studies suggested that neuropeptide Y might act through a protein kinase C (PKC)-dependent mechanism. We directly measured PKC activity in suprachiasmatic nuclei brain slices following application of neuropeptide Y. PKC activity increased 5 min after neuropeptide Y application, with a return to baseline levels 15 min after application. An initial small decrease in PKC activity 1 min after neuropeptide Y application was also observed after control applications of artificial cerebrospinal fluid. Our results support the hypothesis that phase shifts induced by neuropeptide Y involve activation of PKC.

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KW - Neuropeptide Y

KW - Protein kinase C assay

KW - Rhythm

KW - Suprachiasmatic

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Neuropeptide Y activates protein kinase C in hamster suprachiasmatic nuclei brain slices

Abstract

Keywords

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