Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells

Abhishek Chandra, Tatsuo Itakura, Zhi Yang, Tomoki Tamakoshi, Xiao Dong Xue, Bo Wang, Takatoshi Ueki, Kohji Sato, Tadayoshi Uezato, Naoyuki Miura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bone morphogenetic protein (BMP) antagonists regulate the pleiotropic actions of BMPs by binding to BMPs. We previously isolated the Neurogenesin-1 (Ng1) gene and found that Ng1 protein induces neuronal differentiation in the brain. In this study, we found that Ng1 was expressed in the primordial cells of the skeleton and investigated whether Ng1 protein inhibited the BMP action to induce osteoblastic differentiation in C2C12 myoblasts. Interestingly, Ng1 protein inhibited the BMP7-induced alkaline phosphatase activity while it did not inhibit the BMP2-induced activity. All data suggest that Ng1 protein plays an important role in the embryonic bone formation by differentially regulating BMPs.

Original languageEnglish (US)
Pages (from-to)786-791
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number3
DOIs
StatePublished - Jun 9 2006
Externally publishedYes

Fingerprint

Bone Morphogenetic Proteins
Proteins
Myoblasts
Osteogenesis
Skeleton
Alkaline Phosphatase
Brain
Bone
Genes

Keywords

  • Alkaline phosphatase
  • BMP
  • BMP antagonist
  • Differentiation
  • Neurogenesin-1
  • Osteoblast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells. / Chandra, Abhishek; Itakura, Tatsuo; Yang, Zhi; Tamakoshi, Tomoki; Xue, Xiao Dong; Wang, Bo; Ueki, Takatoshi; Sato, Kohji; Uezato, Tadayoshi; Miura, Naoyuki.

In: Biochemical and Biophysical Research Communications, Vol. 344, No. 3, 09.06.2006, p. 786-791.

Research output: Contribution to journalArticle

Chandra, Abhishek ; Itakura, Tatsuo ; Yang, Zhi ; Tamakoshi, Tomoki ; Xue, Xiao Dong ; Wang, Bo ; Ueki, Takatoshi ; Sato, Kohji ; Uezato, Tadayoshi ; Miura, Naoyuki. / Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 344, No. 3. pp. 786-791.
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