Neural isolation of the jejunoileum: Effect on tissue morphometry, mucosal disaccharidase activity, and tissue peptide content

Michael G. Sarr, Mohammad R. Siadati, Jane Bailey, Darlene L. Lucas, Diane R. Roddy, Judith A. Duenes

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to determine the effects of a model of intestinal extrinsic denervation on mucosal structure and function. Six dogs underwent in situ neural isolation of the jejunoileum (Group 2); six other dogs served as operated controls (Group 1), and five nonoperated dogs were naive controls (Group 3). Thirty-centimeter segments of proximal jejunum and distal ileum were excised before (time zero) and at 2 weeks and 8 weeks postoperatively in Groups 1 and 2, while similar regions were removed at time zero in Group 3. Tissues were analyzed for morphology with quantitative morphometry, mucosal disaccharidase activities (sucrase, maltase, and lactase), and tissue content of selected regulatory peptides in transmural, mucosa/submucosa, and muscularis regions. In situ neural isolation had no significant or consistent effects on morphology/morphometry or on mucosal disaccharidase activities. Tissue content of neuropeptide Y decreased markedly (P < 0.002) in all layers of the jejunal and ileal walls, but tissue content of vasoactive inhibitory polypeptide, substance P, cholecystokinin, neurotensin, met-enkephalin, neurokinin A, somatostatin, and calcitonin gene- related peptide demonstrated only minor changes. The physiologic effects of intestinal transplantation (extrinsic denervation and disruption of intrinsic, enteric neural continuity, and lymphatic drainage) have little effect on morphology, mucosal disaccharidase activity, and tissue content of most regulatory peptides. How these minor alterations might affect enteric function, however, needs to be investigated.

Original languageEnglish (US)
Pages (from-to)416-424
Number of pages9
JournalJournal of Surgical Research
Volume61
Issue number2
DOIs
StatePublished - Mar 1996

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Disaccharidases
Mucous Membrane
Peptides
Denervation
Dogs
Neurokinin A
Sucrase
Lactase
Neurotensin
Methionine Enkephalin
Control Groups
alpha-Glucosidases
Calcitonin Gene-Related Peptide
Neuropeptide Y
Cholecystokinin
Jejunum
Substance P
Somatostatin
Ileum
Drainage

ASJC Scopus subject areas

  • Surgery

Cite this

Neural isolation of the jejunoileum : Effect on tissue morphometry, mucosal disaccharidase activity, and tissue peptide content. / Sarr, Michael G.; Siadati, Mohammad R.; Bailey, Jane; Lucas, Darlene L.; Roddy, Diane R.; Duenes, Judith A.

In: Journal of Surgical Research, Vol. 61, No. 2, 03.1996, p. 416-424.

Research output: Contribution to journalArticle

Sarr, Michael G. ; Siadati, Mohammad R. ; Bailey, Jane ; Lucas, Darlene L. ; Roddy, Diane R. ; Duenes, Judith A. / Neural isolation of the jejunoileum : Effect on tissue morphometry, mucosal disaccharidase activity, and tissue peptide content. In: Journal of Surgical Research. 1996 ; Vol. 61, No. 2. pp. 416-424.
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