Nerve growth factor synthesis in vascular smooth muscle

D. Creedon, J. B. Tuttle

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Details of the interdependent, trophic relation between smooth muscle and its neural innervation are not well known despite suggestions that neural influences may contribute significantly to hypertensive and other cardiovascular disease. Vascular smooth muscle is a major target of innervation by neurons of the sympathetic nervous system. Sympathetic neurons depend on a constant supply of the potent neurotrophic peptide nerve growth factor. Nerve growth factor regulates an impressive list of neuronal and perhaps muscle properties, yet its source in vessels and the determinants of its synthesis are not known. We have taken advantage of the cytoarchitecture of the aorta to demonstrate that vascular smooth muscle cells synthesize nerve growth factor. The survival of cultured sympathetic neurons is supported in a nerve growth factor-dependent manner by co-culture with pure rat aortic vascular smooth muscle cells. Furthermore, pure smooth muscle cell cultures contain nerve growth factor-specific messenger RNA. Levels of messenger nucleic acid coding for nerve growth factor in smooth muscle are regulated by contractile agonists (angiotensin II, arginine vasopressin) and the adrenergic agonist phenylephrine. This suggests a link between muscle activity and growth factor production. Secretion of nerve growth factor protein by vascular smooth muscle was measured using a sensitive two-site immunoassay. Secretion is highest during muscle growth. Secretion is elevated by angiotensin II and arginine vasopression but slightly inhibited by phenylephrine. These results suggest that cultured vascular smooth muscle can serve as a useful model in which to study the cellular regulation of trophic factor synthesis in health and disease.

Original languageEnglish (US)
Pages (from-to)730-741
Number of pages12
JournalHypertension
Volume18
Issue number6
StatePublished - 1991
Externally publishedYes

Fingerprint

Nerve Growth Factor
Vascular Smooth Muscle
Smooth Muscle Myocytes
Phenylephrine
Neurons
Angiotensin II
Muscles
Smooth Muscle
Adrenergic Agonists
Arginine Vasopressin
Sympathetic Nervous System
Coculture Techniques
Immunoassay
Nucleic Acids
Arginine
Aorta
Intercellular Signaling Peptides and Proteins
Cardiovascular Diseases
Cell Culture Techniques
Messenger RNA

Keywords

  • growth substances
  • neurons
  • sympathetic nervous system
  • vascular smooth muscle

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Creedon, D., & Tuttle, J. B. (1991). Nerve growth factor synthesis in vascular smooth muscle. Hypertension, 18(6), 730-741.

Nerve growth factor synthesis in vascular smooth muscle. / Creedon, D.; Tuttle, J. B.

In: Hypertension, Vol. 18, No. 6, 1991, p. 730-741.

Research output: Contribution to journalArticle

Creedon, D & Tuttle, JB 1991, 'Nerve growth factor synthesis in vascular smooth muscle', Hypertension, vol. 18, no. 6, pp. 730-741.
Creedon D, Tuttle JB. Nerve growth factor synthesis in vascular smooth muscle. Hypertension. 1991;18(6):730-741.
Creedon, D. ; Tuttle, J. B. / Nerve growth factor synthesis in vascular smooth muscle. In: Hypertension. 1991 ; Vol. 18, No. 6. pp. 730-741.
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