Needle-based confocal endomicroscopy for in vivo histology of intra-abdominal organs: first results in a porcine model (with videos)

Valentin Becker, Michael B. Wallace, Paul Fockens, Stefan von Delius, Timothy A. Woodward, Massimo Raimondo, Rogier P. Voermans, Alexander Meining

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background: Probe-based confocal laser scanning endomicroscopy enables in vivo real time histopathology of the mucosa layer. Recently, a prototype of a new confocal miniprobe has been developed that is small enough to be introduced through a 22-gauge puncture needle. Objective: The aim of this study was to evaluate the feasibility of such needle-based confocal laser scanning endomicroscopy (nCLE) for in vivo histology of various organs in a porcine model. Design: Feasibility study. Setting: Nonsurvival animal experiments with the animals under general anesthesia at three academic centers. Interventions: Ten pigs were examined while they were under general anesthesia. Either EUS-guided organ puncture or natural-orifice transluminal endoscopic surgery (NOTES) procedure was used. The confocal miniprobe was inserted through the 22-gauge needle, and puncture of various intra-abdominal structures and organs was performed (lymph nodes, diaphragm, ovaries, liver, spleen, and pancreas) after intravenous injection of fluorescein (5-10 mL 1% or 2 mL 10% solution). Real-time sequences were recorded. Biopsy specimens were taken for standard histopathology. Main Outcome Measurements and Results: It was technically feasible to introduce the needle-based confocal miniprobe into various organs at the time of EUS or NOTES procedures. The device enabled real-time in vivo collection of images at histologic resolutions and of acceptable image quality from several intra-abdominal organs interrogated. Limitations: Data were assessed in an experimental animal setting and on healthy organs only. Conclusions: Needle-based confocal laser endomicroscopy (CLE) of intra-abdominal organs is feasible in a porcine model. This innovative, minimally invasive technique has the potential to facilitate in vivo histology during EUS punctures or NOTES peritoneoscopy.

Original languageEnglish (US)
Pages (from-to)1260-1266
Number of pages7
JournalGastrointestinal Endoscopy
Volume71
Issue number7
DOIs
StatePublished - Jun 2010
Externally publishedYes

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Needles
Natural Orifice Endoscopic Surgery
Histology
Swine
Punctures
Lasers
General Anesthesia
Feasibility Studies
Diaphragm
Fluorescein
Intravenous Injections
Laparoscopy
Pancreas
Ovary
Mucous Membrane
Spleen
Lymph Nodes
Biopsy
Equipment and Supplies
Liver

ASJC Scopus subject areas

  • Gastroenterology
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Needle-based confocal endomicroscopy for in vivo histology of intra-abdominal organs : first results in a porcine model (with videos). / Becker, Valentin; Wallace, Michael B.; Fockens, Paul; von Delius, Stefan; Woodward, Timothy A.; Raimondo, Massimo; Voermans, Rogier P.; Meining, Alexander.

In: Gastrointestinal Endoscopy, Vol. 71, No. 7, 06.2010, p. 1260-1266.

Research output: Contribution to journalArticle

Becker, Valentin ; Wallace, Michael B. ; Fockens, Paul ; von Delius, Stefan ; Woodward, Timothy A. ; Raimondo, Massimo ; Voermans, Rogier P. ; Meining, Alexander. / Needle-based confocal endomicroscopy for in vivo histology of intra-abdominal organs : first results in a porcine model (with videos). In: Gastrointestinal Endoscopy. 2010 ; Vol. 71, No. 7. pp. 1260-1266.
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AU - Fockens, Paul

AU - von Delius, Stefan

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AB - Background: Probe-based confocal laser scanning endomicroscopy enables in vivo real time histopathology of the mucosa layer. Recently, a prototype of a new confocal miniprobe has been developed that is small enough to be introduced through a 22-gauge puncture needle. Objective: The aim of this study was to evaluate the feasibility of such needle-based confocal laser scanning endomicroscopy (nCLE) for in vivo histology of various organs in a porcine model. Design: Feasibility study. Setting: Nonsurvival animal experiments with the animals under general anesthesia at three academic centers. Interventions: Ten pigs were examined while they were under general anesthesia. Either EUS-guided organ puncture or natural-orifice transluminal endoscopic surgery (NOTES) procedure was used. The confocal miniprobe was inserted through the 22-gauge needle, and puncture of various intra-abdominal structures and organs was performed (lymph nodes, diaphragm, ovaries, liver, spleen, and pancreas) after intravenous injection of fluorescein (5-10 mL 1% or 2 mL 10% solution). Real-time sequences were recorded. Biopsy specimens were taken for standard histopathology. Main Outcome Measurements and Results: It was technically feasible to introduce the needle-based confocal miniprobe into various organs at the time of EUS or NOTES procedures. The device enabled real-time in vivo collection of images at histologic resolutions and of acceptable image quality from several intra-abdominal organs interrogated. Limitations: Data were assessed in an experimental animal setting and on healthy organs only. Conclusions: Needle-based confocal laser endomicroscopy (CLE) of intra-abdominal organs is feasible in a porcine model. This innovative, minimally invasive technique has the potential to facilitate in vivo histology during EUS punctures or NOTES peritoneoscopy.

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