NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1

J. Shi, G. Fung, H. Deng, J. Zhang, Fabienne Fiesel, Wolfdieter Springer, X. Li, H. Luo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Degradation of malfunctional mitochondria by mitophagy is a pivotal component of mitochondrial quality control to maintain cellular homeostasis. Mitochondrial clearance through the PINK1/PARK2 pathway is mediated by autophagic adaptor proteins. Previous studies revealed a significant involvement, but not an absolute requirement for SQSTM1 in PARK2-dependent mitophagy, suggesting that the existence of redundant adaptor proteins may compensate for the loss of SQSTM1. Here we investigated whether NBR1, a functional homolog of SQSTM1, has a role in PARK2-mediated mitophagy, either alone or as a compensatory mechanism. We showed that NBR1 does not appear to be required for mitochondrial clustering following mitochondrial depolarization. Moreover, we demonstrated that deletion of NBR1 alone or in combination with SQSTM1 does not prevent the degradation of damaged mitochondria. Our data suggest that NBR1 is dispensable for PARK2-dependent mitophagy and additional autophagic adaptor proteins, other than NBR1, are responsible for mitochondrial degradation in cells depleted of SQSTM1.

Original languageEnglish (US)
Article numbere1943
JournalCell Death and Disease
Volume6
Issue number10
DOIs
StatePublished - Oct 1 2015

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Mitochondrial Degradation
Mitochondria
Proteins
Quality Control
Cluster Analysis
Homeostasis

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience

Cite this

NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. / Shi, J.; Fung, G.; Deng, H.; Zhang, J.; Fiesel, Fabienne; Springer, Wolfdieter; Li, X.; Luo, H.

In: Cell Death and Disease, Vol. 6, No. 10, e1943, 01.10.2015.

Research output: Contribution to journalArticle

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