Abstract
This is the first report of the direct identification of a HLA-DRB1*03 measles-derived peptide from measles virus infected EBV-transformed B cells. We purified HLA-DR3-peptide complexes from EBV-B cells infected with measles virus (Edmonston strain) and sequenced the HLA-DR3-peptides by mass spectrometry. A class II peptide, derived from a measles phosphoprotein, ASDVETAEGGEIHELLRLQ (P1, residues 179-197), exhibited the capacity to stimulate peripheral blood mononuclear cells to proliferate. Our data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. The approach described herein provides a useful methodology for the future identification of HLA-presented pathogen-derived epitopes using mass spectrometry. The study of cell-mediated immune responses to the measles-derived peptide in immune persons should provide significant insight into the design and development of new vaccines.
Original language | English (US) |
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Pages (from-to) | 495-506 |
Number of pages | 12 |
Journal | Virology |
Volume | 312 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1 2003 |
Keywords
- HLA-DRB1*03
- Lymphocyte proliferation
- Mass spectrometry
- Measles virus
- Measles-derived peptide
ASJC Scopus subject areas
- Virology