Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood

Inna G. Ovsyannikova, Kenneth L. Johnson, Stephen Naylor, David C. Muddiman, Gregory A. Poland

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This is the first report of the direct identification of a HLA-DRB1*03 measles-derived peptide from measles virus infected EBV-transformed B cells. We purified HLA-DR3-peptide complexes from EBV-B cells infected with measles virus (Edmonston strain) and sequenced the HLA-DR3-peptides by mass spectrometry. A class II peptide, derived from a measles phosphoprotein, ASDVETAEGGEIHELLRLQ (P1, residues 179-197), exhibited the capacity to stimulate peripheral blood mononuclear cells to proliferate. Our data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. The approach described herein provides a useful methodology for the future identification of HLA-presented pathogen-derived epitopes using mass spectrometry. The study of cell-mediated immune responses to the measles-derived peptide in immune persons should provide significant insight into the design and development of new vaccines.

Original languageEnglish (US)
Pages (from-to)495-506
Number of pages12
JournalVirology
Volume312
Issue number2
DOIs
StatePublished - Aug 1 2003

Fingerprint

HLA-DRB1 Chains
Measles virus
T-Lymphocytes
Peptides
Measles
HLA-DR3 Antigen
Human Herpesvirus 4
Blood Cells
Mass Spectrometry
B-Lymphocytes
Measles Vaccine
Phosphoproteins
Antigen-Presenting Cells
Epitopes
Vaccines

Keywords

  • HLA-DRB1*03
  • Lymphocyte proliferation
  • Mass spectrometry
  • Measles virus
  • Measles-derived peptide

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood. / Ovsyannikova, Inna G.; Johnson, Kenneth L.; Naylor, Stephen; Muddiman, David C.; Poland, Gregory A.

In: Virology, Vol. 312, No. 2, 01.08.2003, p. 495-506.

Research output: Contribution to journalArticle

Ovsyannikova, Inna G. ; Johnson, Kenneth L. ; Naylor, Stephen ; Muddiman, David C. ; Poland, Gregory A. / Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood. In: Virology. 2003 ; Vol. 312, No. 2. pp. 495-506.
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