Naturally occurring mutations at the acetylcholine receptor binding site independently alter ACh binding and channel gating

Steven M Sine, Xing Ming Shen, Hai Long Wang, Kinji Ohno, Won Yong Lee, Akira Tsujino, Joan Brengmann, Nina Bren, Jiri Vajsar, Andrew G Engel

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

By defining functional defects in a congenital myasthenic syndrome (CMS), we show that two mutant residues, located in a binding site region of the acetylcholine receptor (AChR) epsilon subunit, exert opposite effects on ACh binding and suppress channel gating. Single channel kinetic analysis reveals that the first mutation, εN182Y, increases ACh affinity for receptors in the resting closed state, which promotes sequential occupancy of the binding sites and discloses rate constants for ACh occupancy of the nonmutant αδ site. Studies of the analogous mutation in the δ subunit, δN187Y, disclose rate constants for ACh occupancy of the nonmutant αδ site. The second CMS mutation, εD175N, reduces ACh affinity for receptors in the resting closed state; occupancy of the mutant site still promotes gating because a large difference in affinity is maintained between closed and open states. εD175N impairs overall gating, however, through an effect independent of ACh occupancy. When mapped on a structural model of the AChR binding site, εN182Y localizes to the interface with the α subunit, and eD175 to the entrance of the ACh binding cavity. Both εN182Y and εD175 show state specificity in affecting closed relative to desensitized state affinities, suggesting that the protein chain harboring εN182 and εD175 rearranges in the course of receptor desensitization. The overall results show that key residues at the ACh binding site differentially stabilize the agonist bound to closed, open and desensitized states, and provide a set point for gating of the channel.

Original languageEnglish (US)
Pages (from-to)483-496
Number of pages14
JournalJournal of General Physiology
Volume120
Issue number4
DOIs
StatePublished - Oct 1 2002

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Cholinergic Receptors
Congenital Myasthenic Syndromes
Binding Sites
Mutation
Structural Models
Proteins
1-amino-3-ethyladamantane

Keywords

  • Agonist binding
  • Channel gating
  • Congenital myasthenic syndrome
  • Mutation analysis
  • Single channel kinetics

ASJC Scopus subject areas

  • Physiology

Cite this

Naturally occurring mutations at the acetylcholine receptor binding site independently alter ACh binding and channel gating. / Sine, Steven M; Shen, Xing Ming; Wang, Hai Long; Ohno, Kinji; Lee, Won Yong; Tsujino, Akira; Brengmann, Joan; Bren, Nina; Vajsar, Jiri; Engel, Andrew G.

In: Journal of General Physiology, Vol. 120, No. 4, 01.10.2002, p. 483-496.

Research output: Contribution to journalArticle

Sine, Steven M ; Shen, Xing Ming ; Wang, Hai Long ; Ohno, Kinji ; Lee, Won Yong ; Tsujino, Akira ; Brengmann, Joan ; Bren, Nina ; Vajsar, Jiri ; Engel, Andrew G. / Naturally occurring mutations at the acetylcholine receptor binding site independently alter ACh binding and channel gating. In: Journal of General Physiology. 2002 ; Vol. 120, No. 4. pp. 483-496.
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abstract = "By defining functional defects in a congenital myasthenic syndrome (CMS), we show that two mutant residues, located in a binding site region of the acetylcholine receptor (AChR) epsilon subunit, exert opposite effects on ACh binding and suppress channel gating. Single channel kinetic analysis reveals that the first mutation, εN182Y, increases ACh affinity for receptors in the resting closed state, which promotes sequential occupancy of the binding sites and discloses rate constants for ACh occupancy of the nonmutant αδ site. Studies of the analogous mutation in the δ subunit, δN187Y, disclose rate constants for ACh occupancy of the nonmutant αδ site. The second CMS mutation, εD175N, reduces ACh affinity for receptors in the resting closed state; occupancy of the mutant site still promotes gating because a large difference in affinity is maintained between closed and open states. εD175N impairs overall gating, however, through an effect independent of ACh occupancy. When mapped on a structural model of the AChR binding site, εN182Y localizes to the interface with the α subunit, and eD175 to the entrance of the ACh binding cavity. Both εN182Y and εD175 show state specificity in affecting closed relative to desensitized state affinities, suggesting that the protein chain harboring εN182 and εD175 rearranges in the course of receptor desensitization. The overall results show that key residues at the ACh binding site differentially stabilize the agonist bound to closed, open and desensitized states, and provide a set point for gating of the channel.",
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AB - By defining functional defects in a congenital myasthenic syndrome (CMS), we show that two mutant residues, located in a binding site region of the acetylcholine receptor (AChR) epsilon subunit, exert opposite effects on ACh binding and suppress channel gating. Single channel kinetic analysis reveals that the first mutation, εN182Y, increases ACh affinity for receptors in the resting closed state, which promotes sequential occupancy of the binding sites and discloses rate constants for ACh occupancy of the nonmutant αδ site. Studies of the analogous mutation in the δ subunit, δN187Y, disclose rate constants for ACh occupancy of the nonmutant αδ site. The second CMS mutation, εD175N, reduces ACh affinity for receptors in the resting closed state; occupancy of the mutant site still promotes gating because a large difference in affinity is maintained between closed and open states. εD175N impairs overall gating, however, through an effect independent of ACh occupancy. When mapped on a structural model of the AChR binding site, εN182Y localizes to the interface with the α subunit, and eD175 to the entrance of the ACh binding cavity. Both εN182Y and εD175 show state specificity in affecting closed relative to desensitized state affinities, suggesting that the protein chain harboring εN182 and εD175 rearranges in the course of receptor desensitization. The overall results show that key residues at the ACh binding site differentially stabilize the agonist bound to closed, open and desensitized states, and provide a set point for gating of the channel.

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