Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines

Ying Liu, Ali Rahimian, Sergiy Krylyuk, Tam Vu, Bruno Crulhas, Gulnaz Stybayeva, Meruyert Imanbekova, Dong Sik Shin, Albert Davydov, Alexander Revzin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore, these proteins can be used in diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-γ from T-cells is used for blood-based diagnosis of tuberculosis (TB). Our lab has previously developed an atpamer-based electrochemical biosensor for rapid and sensitive detection of IFN-γ. In this study, we explored the use of silicon nanowires (NWs) as a way to create nanostructured electrodes with enhanced sensitivity for IFN-γ. Si NWs were covered with gold and were further functionalized with thiolated aptamers specific for IFN-γ. Aptamer molecules were designed to form a hairpin and in addition to terminal thiol groups contained redox reporter molecules methylene blue. Binding of analyte to aptamer-modified NWs (termed here nanowire aptasensors) inhibited electron transfer from redox reporters to the electrode and caused electrochemical redox signal to decrease. In a series of experiments we demonstrate that NW aptasensors responded 3× faster and were 2× more sensitive to IFN-γ compared to standard flat electrodes. Most significantly, NW aptasensors allowed detection of IFN-γ from as few as 150 T-cells/mL while ELISA did not pick up signal from the same number of cells. One of the challenges faced by ELISA-based TB diagnostics is poor performance in patients whose T-cell numbers are low, typically HIV patients. Therefore, NW aptasensors developed here may be used in the future for more sensitive monitoring of IFN-γ responses in patients coinfected with HIV/TB.

Original languageEnglish (US)
Pages (from-to)1644-1652
Number of pages9
JournalACS Sensors
Volume2
Issue number11
DOIs
StatePublished - Nov 22 2017

Fingerprint

interferon
Interferons
Nanowires
nanowires
Cytokines
tuberculosis
T-cells
cells
human immunodeficiency virus
infectious diseases
Electrodes
electrodes
proteins
Proteins
Molecules
pathogens
Methylene Blue
methylene blue
Pathogens
Silicon

Keywords

  • aptasensor
  • electrochemical biosensor
  • IFN-γ aptamer
  • nanowire electrode
  • tuberculosis detection

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Cite this

Liu, Y., Rahimian, A., Krylyuk, S., Vu, T., Crulhas, B., Stybayeva, G., ... Revzin, A. (2017). Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines. ACS Sensors, 2(11), 1644-1652. https://doi.org/10.1021/acssensors.7b00486

Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines. / Liu, Ying; Rahimian, Ali; Krylyuk, Sergiy; Vu, Tam; Crulhas, Bruno; Stybayeva, Gulnaz; Imanbekova, Meruyert; Shin, Dong Sik; Davydov, Albert; Revzin, Alexander.

In: ACS Sensors, Vol. 2, No. 11, 22.11.2017, p. 1644-1652.

Research output: Contribution to journalArticle

Liu, Y, Rahimian, A, Krylyuk, S, Vu, T, Crulhas, B, Stybayeva, G, Imanbekova, M, Shin, DS, Davydov, A & Revzin, A 2017, 'Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines', ACS Sensors, vol. 2, no. 11, pp. 1644-1652. https://doi.org/10.1021/acssensors.7b00486
Liu Y, Rahimian A, Krylyuk S, Vu T, Crulhas B, Stybayeva G et al. Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines. ACS Sensors. 2017 Nov 22;2(11):1644-1652. https://doi.org/10.1021/acssensors.7b00486
Liu, Ying ; Rahimian, Ali ; Krylyuk, Sergiy ; Vu, Tam ; Crulhas, Bruno ; Stybayeva, Gulnaz ; Imanbekova, Meruyert ; Shin, Dong Sik ; Davydov, Albert ; Revzin, Alexander. / Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines. In: ACS Sensors. 2017 ; Vol. 2, No. 11. pp. 1644-1652.
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