N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk

W. Zheng, A. C. Deitz, D. R. Campbell, W. Q. Wen, James R Cerhan, T. A. Sellers, A. R. Folsom, D. W. Hein

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

N-Acetyltransferase 1 (NAT1), encoded by the polymorphic NAT1 gene, has been shown to be one of the major enzymes in human breast tissue that activates aromatic and heterocyclic amines. Humans are mainly exposed to these carcinogens through cigarette smoking and consumption of well-done meat. To test the hypothesis that variations in the NAT1 gene are related to breast cancer risk, particularly among women who smoke or consume high levels of well-done meat, a nested case-control study was conducted in a prospective cohort study of 41,837 postmenopausal Iowa women. Information on cigarette smoking and other breast cancer risk factors was obtained at the baseline survey conducted in 1986. DNA samples and information on the consumption of well-done meat were obtained, in the case-control study, from breast cancer cases diagnosed from 1992 to 1994 and a random sample of cancer-free cohort members. Genomic DNA samples obtained from 154 cases and 330 controls were assayed for 11 NAT1 alleles (NAT1 * 3, * 4, * 5, * 10, * 11, * 14, * 15, * 16, * 17, * 19, and * 22). The NAT1 * 4 allele was the predominant allele observed in this study population, accounting for 73.2% (72.4% in cases versus 73.8% in controls) of the total alleles analyzed. Compared to controls, breast cancer cases had a slightly higher frequency of the NAT1 * 10 allele (18.8% in cases versus 17.3% in controls) and a substantially higher frequency of the NAT1 * 11 allele (3.6% versus 1.2%). In multivariate analyses, we found a 30% [95% confidence interval (CI) = 0.8-1.9] elevated risk of breast cancer associated with the NAT1 * 10 allele and a nearly 4- fold (95% CI = 1.5-10.5) elevated risk associated with the NAT1 * 11 allele. The positive association of breast cancer with the NAT1 * 11 allele was more evident among smokers [odds ratio (OR) = 13.2, 95% CI = 1.5-116.0] and those who consumed a high level of red meat (OR = 6.1, 95% CI = 1.1-33.2) or consistently consumed their red meat well done (OR = 5.6, 95% CI = 0.5- 62.7). The association of the NAT1 * 10 allele with breast cancer was mainly confined to former smokers (OR = 3.3, 95% CI = 1.2-9.5). These findings are consistent with a role for the NAT1 gene in the etiology of human breast cancer.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Volume8
Issue number3
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Genetic Polymorphisms
Meat
Smoking
Breast Neoplasms
Alleles
Confidence Intervals
Odds Ratio
N-acetyltransferase 1
Case-Control Studies
Genes
DNA
Smoke
Carcinogens
Amines
Breast
Cohort Studies
Multivariate Analysis
Prospective Studies

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Zheng, W., Deitz, A. C., Campbell, D. R., Wen, W. Q., Cerhan, J. R., Sellers, T. A., ... Hein, D. W. (1999). N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk. Cancer Epidemiology Biomarkers and Prevention, 8(3), 233-239.

N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk. / Zheng, W.; Deitz, A. C.; Campbell, D. R.; Wen, W. Q.; Cerhan, James R; Sellers, T. A.; Folsom, A. R.; Hein, D. W.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 8, No. 3, 03.1999, p. 233-239.

Research output: Contribution to journalArticle

Zheng, W, Deitz, AC, Campbell, DR, Wen, WQ, Cerhan, JR, Sellers, TA, Folsom, AR & Hein, DW 1999, 'N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk', Cancer Epidemiology Biomarkers and Prevention, vol. 8, no. 3, pp. 233-239.
Zheng, W. ; Deitz, A. C. ; Campbell, D. R. ; Wen, W. Q. ; Cerhan, James R ; Sellers, T. A. ; Folsom, A. R. ; Hein, D. W. / N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk. In: Cancer Epidemiology Biomarkers and Prevention. 1999 ; Vol. 8, No. 3. pp. 233-239.
@article{2adc8308518d44bcbbcc6598c43cccbc,
title = "N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk",
abstract = "N-Acetyltransferase 1 (NAT1), encoded by the polymorphic NAT1 gene, has been shown to be one of the major enzymes in human breast tissue that activates aromatic and heterocyclic amines. Humans are mainly exposed to these carcinogens through cigarette smoking and consumption of well-done meat. To test the hypothesis that variations in the NAT1 gene are related to breast cancer risk, particularly among women who smoke or consume high levels of well-done meat, a nested case-control study was conducted in a prospective cohort study of 41,837 postmenopausal Iowa women. Information on cigarette smoking and other breast cancer risk factors was obtained at the baseline survey conducted in 1986. DNA samples and information on the consumption of well-done meat were obtained, in the case-control study, from breast cancer cases diagnosed from 1992 to 1994 and a random sample of cancer-free cohort members. Genomic DNA samples obtained from 154 cases and 330 controls were assayed for 11 NAT1 alleles (NAT1 * 3, * 4, * 5, * 10, * 11, * 14, * 15, * 16, * 17, * 19, and * 22). The NAT1 * 4 allele was the predominant allele observed in this study population, accounting for 73.2{\%} (72.4{\%} in cases versus 73.8{\%} in controls) of the total alleles analyzed. Compared to controls, breast cancer cases had a slightly higher frequency of the NAT1 * 10 allele (18.8{\%} in cases versus 17.3{\%} in controls) and a substantially higher frequency of the NAT1 * 11 allele (3.6{\%} versus 1.2{\%}). In multivariate analyses, we found a 30{\%} [95{\%} confidence interval (CI) = 0.8-1.9] elevated risk of breast cancer associated with the NAT1 * 10 allele and a nearly 4- fold (95{\%} CI = 1.5-10.5) elevated risk associated with the NAT1 * 11 allele. The positive association of breast cancer with the NAT1 * 11 allele was more evident among smokers [odds ratio (OR) = 13.2, 95{\%} CI = 1.5-116.0] and those who consumed a high level of red meat (OR = 6.1, 95{\%} CI = 1.1-33.2) or consistently consumed their red meat well done (OR = 5.6, 95{\%} CI = 0.5- 62.7). The association of the NAT1 * 10 allele with breast cancer was mainly confined to former smokers (OR = 3.3, 95{\%} CI = 1.2-9.5). These findings are consistent with a role for the NAT1 gene in the etiology of human breast cancer.",
author = "W. Zheng and Deitz, {A. C.} and Campbell, {D. R.} and Wen, {W. Q.} and Cerhan, {James R} and Sellers, {T. A.} and Folsom, {A. R.} and Hein, {D. W.}",
year = "1999",
month = "3",
language = "English (US)",
volume = "8",
pages = "233--239",
journal = "Cancer Epidemiology Biomarkers and Prevention",
issn = "1055-9965",
publisher = "American Association for Cancer Research Inc.",
number = "3",

}

TY - JOUR

T1 - N-Acetyltransferase 1 genetic polymorphism, cigarette smoking, well- done meat intake, and breast cancer risk

AU - Zheng, W.

AU - Deitz, A. C.

AU - Campbell, D. R.

AU - Wen, W. Q.

AU - Cerhan, James R

AU - Sellers, T. A.

AU - Folsom, A. R.

AU - Hein, D. W.

PY - 1999/3

Y1 - 1999/3

N2 - N-Acetyltransferase 1 (NAT1), encoded by the polymorphic NAT1 gene, has been shown to be one of the major enzymes in human breast tissue that activates aromatic and heterocyclic amines. Humans are mainly exposed to these carcinogens through cigarette smoking and consumption of well-done meat. To test the hypothesis that variations in the NAT1 gene are related to breast cancer risk, particularly among women who smoke or consume high levels of well-done meat, a nested case-control study was conducted in a prospective cohort study of 41,837 postmenopausal Iowa women. Information on cigarette smoking and other breast cancer risk factors was obtained at the baseline survey conducted in 1986. DNA samples and information on the consumption of well-done meat were obtained, in the case-control study, from breast cancer cases diagnosed from 1992 to 1994 and a random sample of cancer-free cohort members. Genomic DNA samples obtained from 154 cases and 330 controls were assayed for 11 NAT1 alleles (NAT1 * 3, * 4, * 5, * 10, * 11, * 14, * 15, * 16, * 17, * 19, and * 22). The NAT1 * 4 allele was the predominant allele observed in this study population, accounting for 73.2% (72.4% in cases versus 73.8% in controls) of the total alleles analyzed. Compared to controls, breast cancer cases had a slightly higher frequency of the NAT1 * 10 allele (18.8% in cases versus 17.3% in controls) and a substantially higher frequency of the NAT1 * 11 allele (3.6% versus 1.2%). In multivariate analyses, we found a 30% [95% confidence interval (CI) = 0.8-1.9] elevated risk of breast cancer associated with the NAT1 * 10 allele and a nearly 4- fold (95% CI = 1.5-10.5) elevated risk associated with the NAT1 * 11 allele. The positive association of breast cancer with the NAT1 * 11 allele was more evident among smokers [odds ratio (OR) = 13.2, 95% CI = 1.5-116.0] and those who consumed a high level of red meat (OR = 6.1, 95% CI = 1.1-33.2) or consistently consumed their red meat well done (OR = 5.6, 95% CI = 0.5- 62.7). The association of the NAT1 * 10 allele with breast cancer was mainly confined to former smokers (OR = 3.3, 95% CI = 1.2-9.5). These findings are consistent with a role for the NAT1 gene in the etiology of human breast cancer.

AB - N-Acetyltransferase 1 (NAT1), encoded by the polymorphic NAT1 gene, has been shown to be one of the major enzymes in human breast tissue that activates aromatic and heterocyclic amines. Humans are mainly exposed to these carcinogens through cigarette smoking and consumption of well-done meat. To test the hypothesis that variations in the NAT1 gene are related to breast cancer risk, particularly among women who smoke or consume high levels of well-done meat, a nested case-control study was conducted in a prospective cohort study of 41,837 postmenopausal Iowa women. Information on cigarette smoking and other breast cancer risk factors was obtained at the baseline survey conducted in 1986. DNA samples and information on the consumption of well-done meat were obtained, in the case-control study, from breast cancer cases diagnosed from 1992 to 1994 and a random sample of cancer-free cohort members. Genomic DNA samples obtained from 154 cases and 330 controls were assayed for 11 NAT1 alleles (NAT1 * 3, * 4, * 5, * 10, * 11, * 14, * 15, * 16, * 17, * 19, and * 22). The NAT1 * 4 allele was the predominant allele observed in this study population, accounting for 73.2% (72.4% in cases versus 73.8% in controls) of the total alleles analyzed. Compared to controls, breast cancer cases had a slightly higher frequency of the NAT1 * 10 allele (18.8% in cases versus 17.3% in controls) and a substantially higher frequency of the NAT1 * 11 allele (3.6% versus 1.2%). In multivariate analyses, we found a 30% [95% confidence interval (CI) = 0.8-1.9] elevated risk of breast cancer associated with the NAT1 * 10 allele and a nearly 4- fold (95% CI = 1.5-10.5) elevated risk associated with the NAT1 * 11 allele. The positive association of breast cancer with the NAT1 * 11 allele was more evident among smokers [odds ratio (OR) = 13.2, 95% CI = 1.5-116.0] and those who consumed a high level of red meat (OR = 6.1, 95% CI = 1.1-33.2) or consistently consumed their red meat well done (OR = 5.6, 95% CI = 0.5- 62.7). The association of the NAT1 * 10 allele with breast cancer was mainly confined to former smokers (OR = 3.3, 95% CI = 1.2-9.5). These findings are consistent with a role for the NAT1 gene in the etiology of human breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=0032958158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032958158&partnerID=8YFLogxK

M3 - Article

C2 - 10090301

AN - SCOPUS:0032958158

VL - 8

SP - 233

EP - 239

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

IS - 3

ER -