Myosin heavy chain isoform transitions in the developing rat cremasteric muscle correspond with testicular descent

D. R. Vandersteen, D. A. Husmann, R. L. Macken, Gary C Sieck

Research output: Contribution to journalArticle

Abstract

It has been proposed that in the rat, the cremasteric muscle exerts a mechanical force upon the intra-abdominal testis, pulling the testis into the scrotum during development. Since myosin heavy chain (MHC) isoform expression correlates with mechanical properties of muscle fibers, we hypothesized that in the cremasteric muscle, the normal postnatal transitions in MHC isoform expression to adult fast isoforms corresponds temporally with testicular descent. Male Sprague-Dawley rats were euthanized on postnatal days (P) 0, 7, 14, 21, 28 and adults (300 g). The cremasteric muscle was excised. MHC isoform composition was determined by polyacrylamide gel electrophoresis under denaturing conditions. Gels were silver stained, and the relative distribution of each MHC isoform was determined by densitometric analysis. From P0 through P14, the MHC-Neo isoform predominated (>65%). In contrast, in the adult, the MHC-2X isoform predominated (∼80% of total MHC). The half-transition time to adult isoforms occurred at P18, just preceding normal testicular descent on P21. We conclude that with the transition in MHC isoform expression to adult fast MHC isoforms, mechanical performance of the cremasteric muscle improves and that this transition is coincident with testicular descent.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

Myosin Heavy Chains
myosin heavy chains
Muscle
Rats
Protein Isoforms
Muscles
muscles
rats
testes
Testis
scrotum
Scrotum
muscle fibers
silver
Electrophoresis
mechanical properties
polyacrylamide gel electrophoresis
Silver
Sprague Dawley Rats
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Vandersteen, D. R., Husmann, D. A., Macken, R. L., & Sieck, G. C. (1997). Myosin heavy chain isoform transitions in the developing rat cremasteric muscle correspond with testicular descent. FASEB Journal, 11(3).

Myosin heavy chain isoform transitions in the developing rat cremasteric muscle correspond with testicular descent. / Vandersteen, D. R.; Husmann, D. A.; Macken, R. L.; Sieck, Gary C.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Vandersteen, D. R. ; Husmann, D. A. ; Macken, R. L. ; Sieck, Gary C. / Myosin heavy chain isoform transitions in the developing rat cremasteric muscle correspond with testicular descent. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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