Myogenic Akt signaling regulates blood vessel recruitment during myofiber growth

Akihiro Takahashi, Yasuko Kureishi, Jiang Yang, Zhengyu Luo, Kun Guo, Debabrata Mukhopadhyay, Yuri Ivashchenko, Didier Branellec, Kenneth Walsh

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Blood vessel recruitment is an important feature of normal tissue growth. Here, we examined the role of Akt signaling in coordinating angiogenesis with skeletal muscle hypertrophy. Hypertrophy of C2C12 myotubes in response to insulin-like growth factor 1 or insulin and dexamethasone resulted in a marked increase in the secretion of vascular endothelial growth factor (VEGF). Myofiber hypertrophy and hypertrophy-associated VEGF synthesis were specifically inhibited by the transduction of a dominant-negative mutant of the Akt1 serine-threonine protein kinase. Conversely, transduction of constitutively active Akt1 increased myofiber size and led to a robust induction of VEGF protein production. Akt-mediated control of VEGF expression occurred at the level of transcription, and the hypoxia-inducible factor 1 regulatory element was dispensable for this regulation. The activation of Akt1 signaling in normal mouse gastrocnemius muscle was sufficient to promote myofiber hypertrophy, which was accompanied by an increase in circulating and tissue-resident VEGF levels and high capillary vessel densities at focal regions of high Akt transgene expression. In a rabbit hind limb model of vascular insufficiency, intramuscular activation of Akt1 signaling promoted collateral and capillary vessel formation and an accompanying increase in limb perfusion. These data suggest that myogenic Akt signaling controls both fiber hypertrophy and angiogenic growth factor synthesis, illustrating a mechanism through which blood vessel recruitment can be coupled to normal tissue growth.

Original languageEnglish (US)
Pages (from-to)4803-4814
Number of pages12
JournalMolecular and Cellular Biology
Volume22
Issue number13
DOIs
StatePublished - 2002
Externally publishedYes

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Hypertrophy
Blood Vessels
Vascular Endothelial Growth Factor A
Growth
Skeletal Muscle
Extremities
Hypoxia-Inducible Factor 1
Angiogenesis Inducing Agents
Protein-Serine-Threonine Kinases
Skeletal Muscle Fibers
Somatomedins
Transgenes
Dexamethasone
Intercellular Signaling Peptides and Proteins
Perfusion
Insulin
Rabbits
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Myogenic Akt signaling regulates blood vessel recruitment during myofiber growth. / Takahashi, Akihiro; Kureishi, Yasuko; Yang, Jiang; Luo, Zhengyu; Guo, Kun; Mukhopadhyay, Debabrata; Ivashchenko, Yuri; Branellec, Didier; Walsh, Kenneth.

In: Molecular and Cellular Biology, Vol. 22, No. 13, 2002, p. 4803-4814.

Research output: Contribution to journalArticle

Takahashi, A, Kureishi, Y, Yang, J, Luo, Z, Guo, K, Mukhopadhyay, D, Ivashchenko, Y, Branellec, D & Walsh, K 2002, 'Myogenic Akt signaling regulates blood vessel recruitment during myofiber growth', Molecular and Cellular Biology, vol. 22, no. 13, pp. 4803-4814. https://doi.org/10.1128/MCB.22.13.4803-4814.2002
Takahashi, Akihiro ; Kureishi, Yasuko ; Yang, Jiang ; Luo, Zhengyu ; Guo, Kun ; Mukhopadhyay, Debabrata ; Ivashchenko, Yuri ; Branellec, Didier ; Walsh, Kenneth. / Myogenic Akt signaling regulates blood vessel recruitment during myofiber growth. In: Molecular and Cellular Biology. 2002 ; Vol. 22, No. 13. pp. 4803-4814.
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