Mycobacterium tuberculosis 19-kDa lipoprotein promotes neutrophil activation

C. Neufert, Rish Pai, E. H. Noss, M. Berger, W. H. Boom, C. V. Harding

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Certain microbial substances, e.g., LPS, can activate neutrophils or prime them to enhance their response to other activating agents, e.g., fMLP. We investigated the role of the Mycobacterium tuberculosis (MTB) 19-kDa lipoprotein in activation of human neutrophils. MTB 19-kDa lipoprotein initiated phenotypic changes characteristic of neutrophil activation, including down-regulation of CD62 ligand (L-selectin) and up-regulation of CD35 (CR1) and CD11b/CD18 (CR3, Mac-1). In addition, exposure of neutrophils to MTB 19-kDa lipoprotein enhanced the subsequent oxidative burst in response to fMLP as assessed by oxidation of dihydrorhodamine 123 (determined by flow cytometry). LPS also produced these effects with similar kinetics, but an oligodeoxynucleotide containing a CpG motif failed to induce any priming or activation response. Although the effects of LPS required the presence of serum, neutrophil activation by MTB 19-kDa lipoprotein occurred independently of serum factors, suggesting the involvement of different receptors and signaling mechanisms for LPS and MTB 19-kDa lipoprotein. Thus, MTB 19-kDa lipoprotein serves as a pathogen-associated molecular pattern that promotes neutrophil priming and activation.

Original languageEnglish (US)
Pages (from-to)1542-1549
Number of pages8
JournalJournal of Immunology
Volume167
Issue number3
DOIs
StatePublished - Aug 1 2001
Externally publishedYes

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Neutrophil Activation
Mycobacterium tuberculosis
Lipoproteins
Neutrophils
L-Selectin
Respiratory Burst
Oligodeoxyribonucleotides
Serum
Flow Cytometry
Up-Regulation
Down-Regulation
Ligands

ASJC Scopus subject areas

  • Immunology

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Mycobacterium tuberculosis 19-kDa lipoprotein promotes neutrophil activation. / Neufert, C.; Pai, Rish; Noss, E. H.; Berger, M.; Boom, W. H.; Harding, C. V.

In: Journal of Immunology, Vol. 167, No. 3, 01.08.2001, p. 1542-1549.

Research output: Contribution to journalArticle

Neufert, C, Pai, R, Noss, EH, Berger, M, Boom, WH & Harding, CV 2001, 'Mycobacterium tuberculosis 19-kDa lipoprotein promotes neutrophil activation', Journal of Immunology, vol. 167, no. 3, pp. 1542-1549. https://doi.org/10.4049/jimmunol.167.3.1542
Neufert, C. ; Pai, Rish ; Noss, E. H. ; Berger, M. ; Boom, W. H. ; Harding, C. V. / Mycobacterium tuberculosis 19-kDa lipoprotein promotes neutrophil activation. In: Journal of Immunology. 2001 ; Vol. 167, No. 3. pp. 1542-1549.
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AU - Harding, C. V.

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