Mvb12 is a novel member of ESCRT-I involved in cargo selection by the multivesicular body pathway

Andrea J. Oestreich, Brian A. Davies, Johanna A. Payne, David J Katzmann

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The multivesicular body (MVB) sorting pathway impacts a variety of cellular functions in eukaryotic cells. Perhaps the best understood role for the MVB pathway is the degradation of transmembrane proteins within the lysosome. Regulation of cargo selection by this pathway is critically important for normal cell physiology, and recent advances in our understanding of this process have highlighted the endosomal sorting complexes required for transport (ESCRTs) as pivotal players in this reaction. To better understand the mechanisms of cargo selection during MVB sorting, we performed a genetic screen to identify novel factors required for cargo-specific selection by this pathway and identified the Mvb12 protein. Loss of Mvb12 function results in differential defects in the selection of MVB cargoes. A variety of analyses indicate that Mvb12 is a stable member of ESCRT-I, a heterologous complex involved in cargo selection by the MVB pathway. Phenotypes displayed upon loss of Mvb12 are distinct from those displayed by the previously described ESCRT-I subunits (vacuolar protein sorting 23, -28, and -37), suggesting a distinct function than these core subunits. These data support a model in which Mvb12 impacts the selection of MVB cargoes by modulating the cargo recognition capabilities of ESCRT-I.

Original languageEnglish (US)
Pages (from-to)646-657
Number of pages12
JournalMolecular Biology of the Cell
Volume18
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Endosomal Sorting Complexes Required for Transport
Multivesicular Bodies
Cell Physiological Phenomena
Eukaryotic Cells
Protein Transport
Lysosomes
Proteolysis
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Mvb12 is a novel member of ESCRT-I involved in cargo selection by the multivesicular body pathway. / Oestreich, Andrea J.; Davies, Brian A.; Payne, Johanna A.; Katzmann, David J.

In: Molecular Biology of the Cell, Vol. 18, No. 2, 02.2007, p. 646-657.

Research output: Contribution to journalArticle

Oestreich, Andrea J. ; Davies, Brian A. ; Payne, Johanna A. ; Katzmann, David J. / Mvb12 is a novel member of ESCRT-I involved in cargo selection by the multivesicular body pathway. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 2. pp. 646-657.
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