Mutations in the stalk region of the measles virus hemagglutinin inhibit syncytium formation but not virus entry

Matthew K. Ennis, Chunling Hu, Shruthi K. Naik, Louay K. Hallak, Kah-Whye Peng, Stephen J Russell, David M Dingli

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Measles virus (MV) entry requires at least 2 viral proteins, the hemagglutinin (H) and fusion (F) proteins. We describe the rescue and characterization of a measles virus with a specific mutation in the stalk region of H (I98A) that is able to bind normally to cells but infects at a lower rate than the wild type due to a reduction in fusion triggering. The mutant H protein binds to F more avidly than the parent H protein does, and the corresponding virus is more sensitive to inhibition by fusion-inhibitory peptide. We show that after binding of MV to its receptor, H-F dissociation is required for productive infection.

Original languageEnglish (US)
Pages (from-to)10913-10917
Number of pages5
JournalJournal of Virology
Volume84
Issue number20
DOIs
StatePublished - Oct 2010

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ASJC Scopus subject areas

  • Immunology
  • Virology

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