Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach

N. M. Williams, G. Spurlock, Nadine Norton, H. J. Williams, M. L. Hamshere, M. Krawczak, G. Kirov, I. Nikolov, L. Georgieva, S. Jones, A. G. Cardno, M. C. O'Donovan, M. J. Owen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We examined whether variation within six genes from the VCFS critical region at 22q11 (DGSC, Stk22A1, DGSI, Gscl, Slc25A1 and Znf74) confers susceptibility to schizophrenia. We screened the exons and flanking intronic sequence of each gene for mutations in 14 individuals with DSM-IV schizophrenia using DHPLC. All polymorphisms identified were characterised and genotyped in a sample of 184 schizophrenics and matched controls, using novel DNA pooling methods. Of the polymorphisms identified, 17 were located within exons, six were within coding sequence, and two were non-synonymous. Pooled genotyping revealed no differences in the allele frequencies for any polymorphism between cases and controls that met our predefined criterion (P ≤ 0.1). In a complementary approach we also attempted to define the location of a schizophrenia susceptibility locus more precisely by performing association mapping using seven microsatellites spanning the VCFS region with an average inter-marker distance of 450 kb. Conventional X2 analysis of genotypes in 368 cases and 368 controls revealed that none of the markers was significantly associated (P < 0.05) with schizophrenia. However, evidence for significant association (P = 0.003) was obtained for D22S944 when alleles were combined. TDT analysis of D22S944 genotyped in a further 278 cases of schizophrenia and their parents failed to find any overall allele-wise significant transmission disequilibrium (X2 = 18.3, P = 0.17). However, individual analysis of the alleles revealed that allele 12 was excessively non-transmitted and that this almost reached significance when corrected for multiple alleles (X2 = 7.35, P = 0.006, P = 0.078 corrected for 13 alleles).

Original languageEnglish (US)
Pages (from-to)1092-1100
Number of pages9
JournalMolecular Psychiatry
Volume7
Issue number10
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Schizophrenia
Chromosomes
Alleles
Mutation
DNA
Exons
Gene Frequency
Diagnostic and Statistical Manual of Mental Disorders
Microsatellite Repeats
Genes
Genotype

Keywords

  • 22q11
  • LD mapping
  • Polymorphism
  • Schizophrenia
  • VCFS

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Psychiatry and Mental health

Cite this

Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach. / Williams, N. M.; Spurlock, G.; Norton, Nadine; Williams, H. J.; Hamshere, M. L.; Krawczak, M.; Kirov, G.; Nikolov, I.; Georgieva, L.; Jones, S.; Cardno, A. G.; O'Donovan, M. C.; Owen, M. J.

In: Molecular Psychiatry, Vol. 7, No. 10, 2002, p. 1092-1100.

Research output: Contribution to journalArticle

Williams, NM, Spurlock, G, Norton, N, Williams, HJ, Hamshere, ML, Krawczak, M, Kirov, G, Nikolov, I, Georgieva, L, Jones, S, Cardno, AG, O'Donovan, MC & Owen, MJ 2002, 'Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach', Molecular Psychiatry, vol. 7, no. 10, pp. 1092-1100. https://doi.org/10.1038/sj.mp.4001188
Williams, N. M. ; Spurlock, G. ; Norton, Nadine ; Williams, H. J. ; Hamshere, M. L. ; Krawczak, M. ; Kirov, G. ; Nikolov, I. ; Georgieva, L. ; Jones, S. ; Cardno, A. G. ; O'Donovan, M. C. ; Owen, M. J. / Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach. In: Molecular Psychiatry. 2002 ; Vol. 7, No. 10. pp. 1092-1100.
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AU - Hamshere, M. L.

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AU - Kirov, G.

AU - Nikolov, I.

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