Mutation Frequencies But Not Mutant Frequencies in Big Blue® Mice Fit a Poisson Distribution

Hiroshi Nishino, Daniel J Schaid, Victoria L. Buettner, Jan Haavik, Steve S. Sommer

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Transgenic mutation assays generally use mutant Frequencies to estimate mutation frequencies but the degree to which clonal expansion inflates mutant frequencies is largely unknown. Mutant frequency is defined as the fraction of cells carrying mutations in the gene of interest and, according to the standard Big Blue® protocol, is determined by dividing the number of mutant plaques by the total number of plaques screened. Mutation frequency is determined as the fraction of cells carrying definitely independent mutations and therefore requires correction for clonal expansion. Mutant and mutation frequencies were determined for brain, thymus and male germ cells of four mice from two age groups (3- versus 10-month old). The mutant frequency in thymus differed significantly between 3- and 10-month old mice (P < 0.05). By sequencing all mutants, the mutation frequency (i.e., corrected for jackpot mutations) in thymus was determined and was not significantly different between 3- and 10-month old mice. Mutant frequency does not fit a Poisson distribution, but mutation frequency corrected for jackpot mutations is substantially less variable and does fit a Poisson distribution.

Original languageEnglish (US)
Pages (from-to)414-417
Number of pages4
JournalEnvironmental and Molecular Mutagenesis
Volume28
Issue number4
StatePublished - 1996

Fingerprint

Poisson Distribution
Thymus
Poisson distribution
Mutation Rate
mutation
Mutation
Thymus Gland
Assays
Brain
Genes
Cells
Germ Cells
Age Groups
distribution
mutant
germ cell
brain
assay

Keywords

  • Clonol expansion
  • Jackpot mutation
  • lacl
  • Mutant frequency
  • Spontaneous mutation

ASJC Scopus subject areas

  • Genetics
  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology

Cite this

Mutation Frequencies But Not Mutant Frequencies in Big Blue® Mice Fit a Poisson Distribution. / Nishino, Hiroshi; Schaid, Daniel J; Buettner, Victoria L.; Haavik, Jan; Sommer, Steve S.

In: Environmental and Molecular Mutagenesis, Vol. 28, No. 4, 1996, p. 414-417.

Research output: Contribution to journalArticle

Nishino, Hiroshi ; Schaid, Daniel J ; Buettner, Victoria L. ; Haavik, Jan ; Sommer, Steve S. / Mutation Frequencies But Not Mutant Frequencies in Big Blue® Mice Fit a Poisson Distribution. In: Environmental and Molecular Mutagenesis. 1996 ; Vol. 28, No. 4. pp. 414-417.
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