Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer

Akira Yokomizo, Donald J. Tindall, Lynn Hartmann, Robert Brian Jenkins, David I Smith, Wanguo Liu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8%) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalInternational Journal of Oncology
Volume13
Issue number1
StatePublished - Jul 1998

Fingerprint

Ovarian Neoplasms
Mutation
Neoplasms
Multiplex Polymerase Chain Reaction
DNA Sequence Analysis
Point Mutation
Genes
High Pressure Liquid Chromatography
Cell Line

Keywords

  • Ovarian cancer
  • PTEN/MMAC1 gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer. / Yokomizo, Akira; Tindall, Donald J.; Hartmann, Lynn; Jenkins, Robert Brian; Smith, David I; Liu, Wanguo.

In: International Journal of Oncology, Vol. 13, No. 1, 07.1998, p. 101-105.

Research output: Contribution to journalArticle

Yokomizo, Akira ; Tindall, Donald J. ; Hartmann, Lynn ; Jenkins, Robert Brian ; Smith, David I ; Liu, Wanguo. / Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer. In: International Journal of Oncology. 1998 ; Vol. 13, No. 1. pp. 101-105.
@article{0b4aa804e3b145daab2bcd1c5c5b587d,
title = "Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer",
abstract = "A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8{\%}) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.",
keywords = "Ovarian cancer, PTEN/MMAC1 gene",
author = "Akira Yokomizo and Tindall, {Donald J.} and Lynn Hartmann and Jenkins, {Robert Brian} and Smith, {David I} and Wanguo Liu",
year = "1998",
month = "7",
language = "English (US)",
volume = "13",
pages = "101--105",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer

AU - Yokomizo, Akira

AU - Tindall, Donald J.

AU - Hartmann, Lynn

AU - Jenkins, Robert Brian

AU - Smith, David I

AU - Liu, Wanguo

PY - 1998/7

Y1 - 1998/7

N2 - A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8%) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.

AB - A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8%) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.

KW - Ovarian cancer

KW - PTEN/MMAC1 gene

UR - http://www.scopus.com/inward/record.url?scp=0031804873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031804873&partnerID=8YFLogxK

M3 - Article

C2 - 9625810

AN - SCOPUS:0031804873

VL - 13

SP - 101

EP - 105

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 1

ER -