Murine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locus

Xiao Yan Wen, Alexander Keith Stewart, Jennifer Skaug, Ellen Wei, Lap Chee Tsui

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously generated a mouse transgenic line with an insertional mutation designated lpd that demonstrates a phenotype of hypertriglyceridemia and fatty liver. Since the recently identified phosphatidylserine-specific phospholipase A1 (PS-PLA1) demonstrates significant homology to triglyceride lipases, we reasoned that the mouse Ps-pla1 gene may be the disrupted gene within the lpd locus. Using a rat PS-PLA1 cDNA sequence to search the EST database, we identified a mouse EST homolog AA839424. Sequencing analysis of AA839424 revealed a putative Ps-pla1 protein of 456 amino acids with extensive overall structural conservation with human and rat PS-PLA1 and with triglyceride lipases. Conserved sequences in Ps-pla1 include a lipase consensus sequences G×S×G, a catalytic triad, and eight of the ten conserved cysteine residues that are required for tertiary structure. Mouse Ps-pla1 carries a phosphatidylserine-binding motif that is absent in all triglyceride lipases. Using a mouse whole-genome radiation hybrid (WG-RH) mapping panel (T31), we mapped mouse Ps-pla1 to Chromosome (Chr) 16 between genetic markers D16Mit194 and D16Mit38, which is 17.1 cM centromeric to the lpd locus. On the basis of chromosome location, we conclude that Ps-pla1 and lpd are distinct genes in lipid metabolism.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalMammalian Genome
Volume12
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

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Phospholipases A1
Chromosomes, Human, Pair 16
Phosphatidylserines
Lipids
Lipase
Expressed Sequence Tags
Radiation Hybrid Mapping
Genes
Conserved Sequence
Hypertriglyceridemia
Consensus Sequence
Fatty Liver
Genetic Markers
Lipid Metabolism
Transgenic Mice
Cysteine
Complementary DNA
Chromosomes
Genome
Databases

ASJC Scopus subject areas

  • Genetics

Cite this

Murine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locus. / Wen, Xiao Yan; Stewart, Alexander Keith; Skaug, Jennifer; Wei, Ellen; Tsui, Lap Chee.

In: Mammalian Genome, Vol. 12, No. 2, 2001, p. 129-132.

Research output: Contribution to journalArticle

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