Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts

Toshie Sakuma, Jason M. Tonne, Yasuhiro H Ikeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Previously we reported that nuclear export of both unspliced and spliced murine leukemia virus (MLV) transcripts depends on the nuclear export factor (NXF1) pathway. Although the mRNA export complex TREX, which contains Aly/REF, UAP56, and the THO complex, is involved in the NXF1-mediated nuclear export of cellular mRNAs, its contribution to the export of MLV mRNA transcripts remains poorly understood. Here, we studied the involvement of TREX components in the export of MLV transcripts. Depletion of UAP56, but not Aly/REF, reduced the level of both unspliced and spliced viral transcripts in the cytoplasm. Interestingly, depletion of THO components, including THOC5 and THOC7, affected only unspliced viral transcripts in the cytoplasm. Moreover, the RNA immunoprecipitation assay showed that only the unspliced viral transcript interacted with THOC5. These results imply that MLV requires UAP56, THOC5 and THOC7, in addition to NXF1, for nuclear export of viral transcripts. Given that naturally intronless mRNAs, but not bulk mRNAs, require THOC5 for nuclear export, it is plausible that THOC5 plays a key role in the export of unspliced MLV transcripts.

Original languageEnglish (US)
Pages (from-to)1135-1148
Number of pages14
JournalViruses
Volume6
Issue number3
DOIs
StatePublished - Mar 10 2014

Fingerprint

Murine Leukemia Viruses
Cell Nucleus Active Transport
Messenger RNA
Cytoplasm
Immunoprecipitation
RNA

Keywords

  • Gammaretrovirus
  • MLV
  • RNA nuclear export
  • THOC5
  • THOC7
  • TREX
  • UAP56

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts. / Sakuma, Toshie; Tonne, Jason M.; Ikeda, Yasuhiro H.

In: Viruses, Vol. 6, No. 3, 10.03.2014, p. 1135-1148.

Research output: Contribution to journalArticle

Sakuma, Toshie ; Tonne, Jason M. ; Ikeda, Yasuhiro H. / Murine leukemia virus uses TREX components for efficient nuclear export of unspliced viral transcripts. In: Viruses. 2014 ; Vol. 6, No. 3. pp. 1135-1148.
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