Multiplexed detection and quantitation of extracellular vesicle RNA expression using nanostring

Neha Shukla, Irene K. Yan, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Several different types of RNA molecules such as microRNAs (miRNAs) have been detected within extracellular vesicles in the circulation. The detection and potential utility of these as disease biomarkers requires the ability to detect their presence with adequate sensitivity and to quantitate their expression. The potential for circulating miRNA to serve as biomarkers can be evaluated through their detection in association with specific disease states. Multiplexed detection of several miRNA simultaneously can be useful for discovery studies. We describe the analysis of miRNA from biological fluids like plasma and serum using the Nanostring nCounter platform. Assays can be used to quantitate the expression of miRNA using direct detection based on hybridization to target specific color-coded probes followed by counting each color-coded barcode digitally.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages177-185
Number of pages9
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Keywords

  • Biomarkers
  • Extracellular RNA
  • Extracellular vesicles
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shukla, N., Yan, I. K., & Patel, T. C. (2018). Multiplexed detection and quantitation of extracellular vesicle RNA expression using nanostring. In Methods in Molecular Biology (Vol. 1740, pp. 177-185). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_14