Multiplex protein signature for the detection of bladder cancer in voided urine samples

Charles J. Rosser, Shanti Ross, Myron Chang, Yunfeng Dai, Lourdes Mengual, Ge Zhang, Jeongsoon Kim, Virginia Urquidi, Antonio Alcaraz, Steve Goodison

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Purpose: Accurate urine assays for bladder cancer detection would benefit patients and health care systems. Through extensive genomic and proteomic profiling of urine components we previously identified a panel of 8 biomarkers that can facilitate the detection of bladder cancer in voided urine samples. In this study we confirmed this diagnostic molecular signature in a diverse multicenter cohort. Materials and Methods: We performed a case-control, phase II study in which we analyzed voided urine from 102 subjects with bladder cancer and 206 with varying urological disorders. The urinary concentration of 8 biomarkers (IL-8, MMP-9 and 10, PAI-1, VEGF, ANG, CA9 and APOE) was assessed by enzymelinked immunosorbent assay. Diagnostic performance of the panel of tested biomarkers was evaluated using ROCs and descriptive statistical values, eg sensitivity and specificity. Results: Seven of the 8 urine biomarkers were increased in subjects with bladder cancer relative to those without bladder cancer. The 7 biomarkers were assessed in a new model, which had an AUROC of 0.88 (95% CI 0.84-0.93), and 74% sensitivity and 90% specificity. In contrast, the sensitivity of voided urine cytology and the UroVysion® cytogenetic test in this cohort was 39% and 54%, respectively. Study limitations include analysis performed on banked urine samples and the lack of voided urine cytology and cytogenetic test data on controls. Conclusions: The study provides further evidence that the reported panel of diagnostic biomarkers can reliably achieve the noninvasive detection of bladder cancer with higher sensitivity than currently available urine based assays.

Original languageEnglish (US)
Pages (from-to)2257-2262
Number of pages6
JournalJournal of Urology
Volume190
Issue number6
DOIs
StatePublished - Dec 2013

Keywords

  • Biological markers
  • Diagnosis
  • Molecular biology
  • Urinary bladder neoplasms
  • Urine

ASJC Scopus subject areas

  • Urology

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  • Cite this

    Rosser, C. J., Ross, S., Chang, M., Dai, Y., Mengual, L., Zhang, G., Kim, J., Urquidi, V., Alcaraz, A., & Goodison, S. (2013). Multiplex protein signature for the detection of bladder cancer in voided urine samples. Journal of Urology, 190(6), 2257-2262. https://doi.org/10.1016/j.juro.2013.06.011