Multiplex polymerase chain reaction.

L. J. Burgart, R. A. Robinson, M. J. Heller, W. W. Wilke, O. K. Iakoubova, J. C. Cheville

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced, conserving expensive reagents and decreasing possible contamination. Multiplex PCR allows for an assay of the gene of interest and assures that the amplification process proceeds as expected with the use of a companion control genome primer set. Multiplex PCR is useful in assaying DNA extracted from samples of immunocompromised patients in which more than one infectious agent may be suspected such as simultaneous EBV and CMV detection. Multiplex PCR offers many advantages over single reaction PCR and has been found to be an useful adjunct in our laboratory.

Original languageEnglish (US)
Pages (from-to)320-323
Number of pages4
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume5
Issue number3
StatePublished - May 1992
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Burgart, L. J., Robinson, R. A., Heller, M. J., Wilke, W. W., Iakoubova, O. K., & Cheville, J. C. (1992). Multiplex polymerase chain reaction. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 5(3), 320-323.