Multiple divergent mRNAs code for a single human calmodulin

R. Fischer, M. Koller, M. Flura, S. Mathews, M. A. Strehler-Page, J. Krebs, J. T. Penniston, E. Carafoli, E. E. Strehler

Research output: Contribution to journalArticle

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Abstract

The isolation of a novel complementary DNA (cDNA) clone coding for human calmodulin (CaM) is reported. Although it encodes a protein indistinguishable from the only known higher vertebrate calmodulin, its nucleotide sequence varies extensively from that of two previously reported human CaM cDNAs (Wawrzynczak and Perham, 1984; SenGupta et al., 1987). Only 82 and 81% identity, respectively, is found between the newly isolated and the two known human mRNAs in their coding regions. No striking homology is present in their noncoding regions. Coding usage in the three CaM mRNAs is also surprisingly divergent. A 2.3-kilobase mRNA corresponding to the newly isolated clone is expressed to varying extents in several human tissues, together with an approximately 0.8-kilobase mRNA species presumably arising from alternative polyadenylation of the same primary transcript. The results indicate that the human genome contains at least three divergent CaM genes that are under selective pressure to encode an identical protein while maintaining maximally divergent nucleotide sequences. Partial characterization of a genomic clone specifying the 3' portion of the newly identified CaM mRNA shows that this gene contains introns at identical positions as the previously characterized bona fide vertebrate CaM genes. Evolutionary implications of the presence of a CaM multigene family are discussed.

Original languageEnglish (US)
Pages (from-to)17055-17062
Number of pages8
JournalJournal of Biological Chemistry
Volume263
Issue number32
StatePublished - 1988
Externally publishedYes

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Calmodulin
Messenger RNA
Genes
Clone Cells
Vertebrates
Nucleotides
Complementary DNA
Polyadenylation
Human Genome
Multigene Family
Introns
Proteins
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fischer, R., Koller, M., Flura, M., Mathews, S., Strehler-Page, M. A., Krebs, J., ... Strehler, E. E. (1988). Multiple divergent mRNAs code for a single human calmodulin. Journal of Biological Chemistry, 263(32), 17055-17062.

Multiple divergent mRNAs code for a single human calmodulin. / Fischer, R.; Koller, M.; Flura, M.; Mathews, S.; Strehler-Page, M. A.; Krebs, J.; Penniston, J. T.; Carafoli, E.; Strehler, E. E.

In: Journal of Biological Chemistry, Vol. 263, No. 32, 1988, p. 17055-17062.

Research output: Contribution to journalArticle

Fischer, R, Koller, M, Flura, M, Mathews, S, Strehler-Page, MA, Krebs, J, Penniston, JT, Carafoli, E & Strehler, EE 1988, 'Multiple divergent mRNAs code for a single human calmodulin', Journal of Biological Chemistry, vol. 263, no. 32, pp. 17055-17062.
Fischer R, Koller M, Flura M, Mathews S, Strehler-Page MA, Krebs J et al. Multiple divergent mRNAs code for a single human calmodulin. Journal of Biological Chemistry. 1988;263(32):17055-17062.
Fischer, R. ; Koller, M. ; Flura, M. ; Mathews, S. ; Strehler-Page, M. A. ; Krebs, J. ; Penniston, J. T. ; Carafoli, E. ; Strehler, E. E. / Multiple divergent mRNAs code for a single human calmodulin. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 32. pp. 17055-17062.
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AU - Krebs, J.

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AU - Strehler, E. E.

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N2 - The isolation of a novel complementary DNA (cDNA) clone coding for human calmodulin (CaM) is reported. Although it encodes a protein indistinguishable from the only known higher vertebrate calmodulin, its nucleotide sequence varies extensively from that of two previously reported human CaM cDNAs (Wawrzynczak and Perham, 1984; SenGupta et al., 1987). Only 82 and 81% identity, respectively, is found between the newly isolated and the two known human mRNAs in their coding regions. No striking homology is present in their noncoding regions. Coding usage in the three CaM mRNAs is also surprisingly divergent. A 2.3-kilobase mRNA corresponding to the newly isolated clone is expressed to varying extents in several human tissues, together with an approximately 0.8-kilobase mRNA species presumably arising from alternative polyadenylation of the same primary transcript. The results indicate that the human genome contains at least three divergent CaM genes that are under selective pressure to encode an identical protein while maintaining maximally divergent nucleotide sequences. Partial characterization of a genomic clone specifying the 3' portion of the newly identified CaM mRNA shows that this gene contains introns at identical positions as the previously characterized bona fide vertebrate CaM genes. Evolutionary implications of the presence of a CaM multigene family are discussed.

AB - The isolation of a novel complementary DNA (cDNA) clone coding for human calmodulin (CaM) is reported. Although it encodes a protein indistinguishable from the only known higher vertebrate calmodulin, its nucleotide sequence varies extensively from that of two previously reported human CaM cDNAs (Wawrzynczak and Perham, 1984; SenGupta et al., 1987). Only 82 and 81% identity, respectively, is found between the newly isolated and the two known human mRNAs in their coding regions. No striking homology is present in their noncoding regions. Coding usage in the three CaM mRNAs is also surprisingly divergent. A 2.3-kilobase mRNA corresponding to the newly isolated clone is expressed to varying extents in several human tissues, together with an approximately 0.8-kilobase mRNA species presumably arising from alternative polyadenylation of the same primary transcript. The results indicate that the human genome contains at least three divergent CaM genes that are under selective pressure to encode an identical protein while maintaining maximally divergent nucleotide sequences. Partial characterization of a genomic clone specifying the 3' portion of the newly identified CaM mRNA shows that this gene contains introns at identical positions as the previously characterized bona fide vertebrate CaM genes. Evolutionary implications of the presence of a CaM multigene family are discussed.

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